RecJ

• Description: single-strand DNA-specific exonuclease
  
  

Gene name	yrvE
Synonyms	recJ
Essential	no
Product	single-strand DNA-specific exonuclease
Function	DNA repair
Gene expression levels in SubtiExpress: yrvE
Interactions involving this protein in SubtInteract: YrvE
Metabolic function and regulation of this protein in SubtiPathways: yrvE
MW, pI	87 kDa, 5.181
Gene length, protein length	2358 bp, 786 aa
Immediate neighbours	apt, yrvD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

stringent response, Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU27620

Database entries

• BsubCyc: BSU27620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: recJ family (according to Swiss-Prot)

• Paralogous protein(s): YorK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SsbA-YrvE PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27620

• Structure:

• UniProt: O32044

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yrvE-apt PubMed

• Expression browser: yrvE PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:
  • GP895 (recJ::kan), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Hanjing Yang, Cameron Sikavi, Katherine Tran, Shauna M McGillivray, Victor Nizet, Madeline Yung, Aileen Chang, Jeffrey H Miller
Papillation in Bacillus anthracis colonies: a tool for finding new mutators.
Mol Microbiol: 2011, 79(5);1276-93
[PubMed:21205011] [WorldCat.org] [DOI] (I p)

Audrey Costes, François Lecointe, Stephen McGovern, Sophie Quevillon-Cheruel, Patrice Polard
The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.
PLoS Genet: 2010, 6(12);e1001238
[PubMed:21170359] [WorldCat.org] [DOI] (I e)

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

V A Sutera, E S Han, L A Rajman, S T Lovett
Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs.
J Bacteriol: 1999, 181(19);6098-102
[PubMed:10498723] [WorldCat.org] [DOI] (P p)