CheY

• Description: two-component response regulator, modulation of flagellar switch bias
  
  

Gene name	cheY
Synonyms	cheB
Essential	no
Product	two-component response regulator
Function	modulation of flagellar switch bias
Gene expression levels in SubtiExpress: cheY
Interactions involving this protein in SubtInteract: CheY
MW, pI	13 kDa, 4.746
Gene length, protein length	360 bp, 120 aa
Immediate neighbours	fliY, fliZ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, motility and chemotaxis, phosphoproteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16330

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16330

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YneI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated by CheA on an Asp residue
  • dephosphorylated by CheC PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • CheY-P-CheC PubMed
  • CheA-CheY

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16330

• Structure: 1TMY (CheY from Thermotoga maritima)

• UniProt: P24072

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheY PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • in minimal medium, CheY is present with 7,100 +/- 1,000 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1164 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2117 PubMed

Biological materials

• Mutant:
  • 1A798 ( cheY::cat), PubMed, available at BGSC
  • DS6870 (marker-less in NCIB3610) PubMed
  • TB191 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB207 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: