CheD

• Description: chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity
  
  

Gene name	cheD
Synonyms	ylxK
Essential	no
Product	protein deaminase, CheC activity modulator
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: cheD
Interactions involving this protein in SubtInteract: CheD
MW, pI	17 kDa, 9.347
Gene length, protein length	498 bp, 166 aa
Immediate neighbours	cheC, sigD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16460

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16460

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds chemoreceptors and increases their ability to activate the kinase activity of CheA PubMed
  • Protein L-glutamine + H₂O = protein L-glutamate + NH₃ (according to Swiss-Prot)
  • deaminates Gln-586, Gln-593, and Gln594 in McpA PubMed
  • deaminates Gln-609 in McpC PubMed

• Protein family: cheD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CheC-CheD PubMed, this interaction is enhanced by CheY-P PubMed
  • CheD-McpA, deamination of Gln-586, Gln-593 and Gln-594 in McpA PubMed
  • CheD-McpC, deamination of Gln-609 in McpC PubMed
  • McpB-CheD to increase the ability of McpB to activate CheA PubMed

• Localization:

Database entries

• BsubCyc: BSU16460

• Structure:

• UniProt: P40404

• KEGG entry: [3]

• E.C. number: 3.5.1.44

Expression and regulation

• Operon: part of the fla-che operon
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheD PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • in minimal medium, CheD is present with 1,200 +/- 250 molecules per cell PubMed

Biological materials

• Mutant:
  • 1A861 ( cheD::cat), PubMed, available at BGSC
  • 1A863 ( cheD::cat), PubMed, available at BGSC
  • DS6868 (marker-less in NCIB3610) PubMed
  • TB190 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB206 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: