Difference between revisions of "CheC"

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(Expression and regulation)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A862 (no resistance), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A862&Search=1A862 BGSC]
 
** 1A862 (no resistance), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A862&Search=1A862 BGSC]
 +
** DS6867 (marker-less in NCIB3610) {{PubMed|25313396}}
 +
** TB189 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
 +
** TB205 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
<pubmed>7893679,14993307,18774298,8866475,18990184,17908686,14749334,11722727,9194713, 14651647, 9657996,8157612, 15175317,17609139, 16469702, 20080618,17675386,20133180 17850253 21515776 23226535 24386445</pubmed>
+
<pubmed> 25313396, 26122431, 7893679,14993307,18774298,8866475,18990184,17908686,14749334,11722727,9194713, 14651647, 9657996,8157612, 15175317,17609139, 16469702, 20080618,17675386,20133180 17850253 21515776 23226535 24386445</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:53, 2 July 2015

  • Description: control of chemotaxis by interacting with CheD, CheY-P phosphatase, inhibition of CheR-mediated methylation of MCPs

Gene name cheC
Synonyms ylxJ
Essential no
Product CheY-P phosphatase
Function motility and chemotaxis
Gene expression levels in SubtiExpress: cheC
Interactions involving this protein in SubtInteract: CheC
MW, pI 22 kDa, 4.035
Gene length, protein length 627 bp, 209 aa
Immediate neighbours cheW, cheD
Sequences Protein DNA DNA_with_flanks
Genetic context
CheC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CheC expression.png















Categories containing this gene/protein

protein modification, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16450

Phenotypes of a mutant

  • not essential for pellicle biofilm formation and mutant has similar fitness to the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • CheY-P phosphatase
    • controls binding of CheD to chemoreceptors in response to the levels of CheY-P PubMed
  • Protein family: cheC family (according to Swiss-Prot)
  • Paralogous protein(s):FliY

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: interaction with CheD enhances phosphatase activity towards CheY-P PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • in minimal medium, CheC is present with 770 +/- 330 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 152 PubMed

Biological materials

  • Mutant:
    • 1A862 (no resistance), PubMed, available at BGSC
    • DS6867 (marker-less in NCIB3610) PubMed
    • TB189 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB205 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References