CheA

• Description: two-component sensor kinase, chemotactic signal modulator
  
  

Gene name	cheA
Synonyms	cheN
Essential	no
Product	two-component sensor kinase
Function	chemotactic signal modulator
Gene expression levels in SubtiExpress: cheA
Interactions involving this protein in SubtInteract: CheA
MW, pI	74 kDa, 4.452
Gene length, protein length	2013 bp, 671 aa
Immediate neighbours	cheB, cheW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, motility and chemotaxis, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU16430

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU16430

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of CheY and CheB PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • phosphorylation of CheA is stimulated by interaction with the complex of deaminated McpC-CheD PubMed
  • the kinase activity of CheA is activated by chemoreceptors bound to CheD PubMed

• Interactions:
  • CheA-McpB PubMed
  • CheA-CheC PubMed
  • CheA-CheY PubMed
  • CheA-CheW PubMed

• Localization:
  • membrane (according to Swiss-Prot)
  • localizes to the cell poles PubMed

Database entries

• BsubCyc: BSU16430

• Structure:

• UniProt: P29072

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: part of the fla-che operon
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Expression browser: cheA PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation: see fla-che operon

• Regulatory mechanism: see fla-che operon

• Additional information:
  • in minimal medium, CheA is present with 2,600 +/- 560 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 705 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1580 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 483 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 188 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 62 PubMed

Biological materials

• Mutant:
  • 1A797 ( cheA::cat), PubMed, available at BGSC
  • DS6887 (marker-less in NCIB3610) PubMed
  • TB197 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB213 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Daniel Kearns