BceB

• Description: ABC transporter (permease) for the export of bacitracin, plectasin, mersacidin and actagardine, sensory component of the Bce regulatory & detoxification system
  
  

Gene name	bceB
Synonyms	ytsD
Essential	no
Product	bacitracin ABC transporter (permease)
Function	export of toxic peptides
Gene expression levels in SubtiExpress: bceB
Interactions involving this protein in SubtInteract: BceB
MW, pI	72 kDa, 9.79
Gene length, protein length	1938 bp, 646 aa
Immediate neighbours	yttA, bceA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

BceR regulon

The gene

Basic information

• Locus tag: BSU30370

Database entries

• BsubCyc: BSU30370

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds bacitracin, K^D: 60 nM PubMed
  • inhibits BceS autophosphorylation activity in the absence of bacitracin PubMed

• Protein family: ABC-4 integral membrane protein family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • the activity of the BceA-BceB ABC transporter is inhibited by heptaprenyl pyrophosphate which accumulates in a ytpB mutant PubMed

• Interactions:
  • BceA-BceB PubMed
  • BceB-BceS, in the absence of bacitracin PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU30370

• Structure:

• UniProt: O34741

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: bceA-bceB PubMed

• Expression browser: bceB PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine (BceR) PubMed

• Regulatory mechanism:
  • BceR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sebastian Dintner, Ralf Heermann, Chong Fang, Kirsten Jung, Susanne Gebhard
A sensory complex consisting of an ATP-binding cassette transporter and a two-component regulatory system controls bacitracin resistance in Bacillus subtilis.
J Biol Chem: 2014, 289(40);27899-910
[PubMed:25118291] [WorldCat.org] [DOI] (I p)

Anthony W Kingston, Heng Zhao, Gregory M Cook, John D Helmann
Accumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: implications for the mechanism of resistance mediated by the BceAB transporter.
Mol Microbiol: 2014, 93(1);37-49
[PubMed:24806199] [WorldCat.org] [DOI] (I p)

Felix Kallenberg, Sebastian Dintner, Roland Schmitz, Susanne Gebhard
Identification of regions important for resistance and signalling within the antimicrobial peptide transporter BceAB of Bacillus subtilis.
J Bacteriol: 2013, 195(14);3287-97
[PubMed:23687272] [WorldCat.org] [DOI] (I p)

Stéphanie Coumes-Florens, Céline Brochier-Armanet, Annick Guiseppi, François Denizot, Maryline Foglino
A new highly conserved antibiotic sensing/resistance pathway in firmicutes involves an ABC transporter interplaying with a signal transduction system.
PLoS One: 2011, 6(1);e15951
[PubMed:21283517] [WorldCat.org] [DOI] (I e)

Anna Staroń, Dora Elisabeth Finkeisen, Thorsten Mascher
Peptide antibiotic sensing and detoxification modules of Bacillus subtilis.
Antimicrob Agents Chemother: 2011, 55(2);515-25
[PubMed:21078927] [WorldCat.org] [DOI] (I p)

Jing Ouyang, Xiao-Lin Tian, Jennifer Versey, Alexander Wishart, Yung-Hua Li
The BceABRS four-component system regulates the bacitracin-induced cell envelope stress response in Streptococcus mutans.
Antimicrob Agents Chemother: 2010, 54(9);3895-906
[PubMed:20606066] [WorldCat.org] [DOI] (I p)

Eva Rietkötter, Diana Hoyer, Thorsten Mascher
Bacitracin sensing in Bacillus subtilis.
Mol Microbiol: 2008, 68(3);768-85
[PubMed:18394148] [WorldCat.org] [DOI] (I p)

Remi Bernard, Annick Guiseppi, Marc Chippaux, Maryline Foglino, François Denizot
Resistance to bacitracin in Bacillus subtilis: unexpected requirement of the BceAB ABC transporter in the control of expression of its own structural genes.
J Bacteriol: 2007, 189(23);8636-42
[PubMed:17905982] [WorldCat.org] [DOI] (I p)

Thorsten Mascher, Neil G Margulis, Tao Wang, Rick W Ye, John D Helmann
Cell wall stress responses in Bacillus subtilis: the regulatory network of the bacitracin stimulon.
Mol Microbiol: 2003, 50(5);1591-604
[PubMed:14651641] [WorldCat.org] [DOI] (P p)

Remi Bernard, Pascale Joseph, Annick Guiseppi, Marc Chippaux, François Denizot
YtsCD and YwoA, two independent systems that confer bacitracin resistance to Bacillus subtilis.
FEMS Microbiol Lett: 2003, 228(1);93-7
[PubMed:14612242] [WorldCat.org] [DOI] (P p)

Reiko Ohki, Giyanto, Kozue Tateno, Waka Masuyama, Shigeki Moriya, Kazuo Kobayashi, Naotake Ogasawara
The BceRS two-component regulatory system induces expression of the bacitracin transporter, BceAB, in Bacillus subtilis.
Mol Microbiol: 2003, 49(4);1135-44
[PubMed:12890034] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)