YidC2

• Description: Sec-independent membrane protein translocase, required for development of genetic competence
  
  

Gene name	yidC2
Synonyms	yqjG, oxaAB
Essential	no
Product	Sec-independent membrane protein translocase
Function	membrane insertion of proteins and protein secretion
MW, pI	30 kDa, 9.903
Gene length, protein length	825 bp, 275 aa
Immediate neighbours	mifM, yqjF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein secretion, genetic competence, membrane proteins

This gene is a member of the following regulons

MifM regulon

The gene

Basic information

• Locus tag: BSU23890

Database entries

• BsubCyc: BSU23890

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: YidC/Oxa1/Alb3 family PubMed

• Paralogous protein(s): SpoIIIJ, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane PubMed

Database entries

• BsubCyc: BSU23890

• Structure:

• UniProt: P54544

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mifM-yidC2 PubMed

• Expression browser: yidC2 PubMed

• Sigma factor:

• Regulation:
  • repressed by large amounts of SpoIIIJ, induced by small amounts of SpoIIIJ (MifM) PubMed

• Regulatory mechanism:
  • MifM: "attenuation mechanism", an mRNA hairpin of the yqjG transcript unfold upon translation arrest of MifM at decreased SpoIIIJ levels PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ross E Dalbey, Peng Wang, Jan Maarten van Dijl
Membrane proteases in the bacterial protein secretion and quality control pathway.
Microbiol Mol Biol Rev: 2012, 76(2);311-30
[PubMed:22688815] [WorldCat.org] [DOI] (I p)

Soledad Funes, Frank Kauff, Eli O van der Sluis, Martin Ott, Johannes M Herrmann
Evolution of YidC/Oxa1/Alb3 insertases: three independent gene duplications followed by functional specialization in bacteria, mitochondria and chloroplasts.
Biol Chem: 2011, 392(1-2);13-9
[PubMed:21194367] [WorldCat.org] [DOI] (I p)

Peng Wang, Ross E Dalbey
Inserting membrane proteins: the YidC/Oxa1/Alb3 machinery in bacteria, mitochondria, and chloroplasts.
Biochim Biophys Acta: 2011, 1808(3);866-75
[PubMed:20800571] [WorldCat.org] [DOI] (P p)

Martin van der Laan, Nico P Nouwen, Arnold J M Driessen
YidC--an evolutionary conserved device for the assembly of energy-transducing membrane protein complexes.
Curr Opin Microbiol: 2005, 8(2);182-7
[PubMed:15802250] [WorldCat.org] [DOI] (P p)

Original publications

Daniel Sohmen, Shinobu Chiba, Naomi Shimokawa-Chiba, C Axel Innis, Otto Berninghausen, Roland Beckmann, Koreaki Ito, Daniel N Wilson
Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling.
Nat Commun: 2015, 6;6941
[PubMed:25903689] [WorldCat.org] [DOI] (I e)

Naomi Shimokawa-Chiba, Kaoru Kumazaki, Tomoya Tsukazaki, Osamu Nureki, Koreaki Ito, Shinobu Chiba
Hydrophilic microenvironment required for the channel-independent insertase function of YidC protein.
Proc Natl Acad Sci U S A: 2015, 112(16);5063-8
[PubMed:25855636] [WorldCat.org] [DOI] (I p)

Yuanyuan Chen, Raunak Soman, Sri Karthika Shanmugam, Andreas Kuhn, Ross E Dalbey
The role of the strictly conserved positively charged residue differs among the Gram-positive, Gram-negative, and chloroplast YidC homologs.
J Biol Chem: 2014, 289(51);35656-67
[PubMed:25359772] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Koreaki Ito
MifM monitors total YidC activities of Bacillus subtilis, including that of YidC2, the target of regulation.
J Bacteriol: 2015, 197(1);99-107
[PubMed:25313395] [WorldCat.org] [DOI] (I p)

Yanping Geng, Jeanine de Keyzer, Dirk-Jan Scheffers, Arnold J M Driessen
Defining the region of Bacillus subtilis SpoIIIJ that is essential for its sporulation-specific function.
J Bacteriol: 2014, 196(7);1318-24
[PubMed:24443530] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Koreaki Ito
Multisite ribosomal stalling: a unique mode of regulatory nascent chain action revealed for MifM.
Mol Cell: 2012, 47(6);863-72
[PubMed:22864117] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Manfred J Saller, Andreas Otto, Greetje A Berrelkamp-Lahpor, Dörte Becher, Michael Hecker, Arnold J M Driessen
Bacillus subtilis YqjG is required for genetic competence development.
Proteomics: 2011, 11(2);270-82
[PubMed:21204254] [WorldCat.org] [DOI] (I p)

Shinobu Chiba, Anne Lamsa, Kit Pogliano
A ribosome-nascent chain sensor of membrane protein biogenesis in Bacillus subtilis.
EMBO J: 2009, 28(22);3461-75
[PubMed:19779460] [WorldCat.org] [DOI] (I p)

Manfred J Saller, Fabrizia Fusetti, Arnold J M Driessen
Bacillus subtilis SpoIIIJ and YqjG function in membrane protein biogenesis.
J Bacteriol: 2009, 191(21);6749-57
[PubMed:19717609] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Xin Jiang, Kit Pogliano
Localization of translocation complex components in Bacillus subtilis: enrichment of the signal recognition particle receptor at early sporulation septa.
J Bacteriol: 2005, 187(14);5000-2
[PubMed:15995216] [WorldCat.org] [DOI] (P p)

Harold Tjalsma, Sierd Bron, Jan Maarten van Dijl
Complementary impact of paralogous Oxa1-like proteins of Bacillus subtilis on post-translocational stages in protein secretion.
J Biol Chem: 2003, 278(18);15622-32
[PubMed:12586834] [WorldCat.org] [DOI] (P p)

Takako Murakami, Koki Haga, Michio Takeuchi, Tsutomu Sato
Analysis of the Bacillus subtilis spoIIIJ gene and its Paralogue gene, yqjG.
J Bacteriol: 2002, 184(7);1998-2004
[PubMed:11889108] [WorldCat.org] [DOI] (P p)