Difference between revisions of "NrgB"
(→Biological materials) |
|||
| Line 1: | Line 1: | ||
| − | * '''Description:''' nitrogen-regulated PII | + | * '''Description:''' nitrogen-regulated PII protein <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || nitrogen-regulated PII | + | |style="background:#ABCDEF;" align="center"| '''Product''' || nitrogen-regulated PII protein |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of ammonium uptake | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of ammonium uptake | ||
| Line 64: | Line 64: | ||
=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| Line 99: | Line 97: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.ebi.ac.uk/pdbe-srv/view/entry/2nuu/summary 2NUU] (the complex with [[NrgA]], from ''E. coli'') {{PubMed|17220269}} | + | * '''Structure:''' |
| + | ** [http://pdb.org/pdb/search/structidSearch.do?structureId=4r25 4R25] {{PubMed|25691471}} | ||
| + | ** [http://www.ebi.ac.uk/pdbe-srv/view/entry/2nuu/summary 2NUU] (the complex with [[NrgA]], from ''E. coli'') {{PubMed|17220269}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q07428 Q07428] | * '''UniProt:''' [http://www.uniprot.org/uniprot/Q07428 Q07428] | ||
| Line 149: | Line 149: | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>23010999 12823818,,8799114,17001076 , 14600241 ,8282685 ,1670935 17220269 23535029 23818625 21435182</pubmed> | + | <pubmed>23010999 12823818,,8799114,17001076 , 14600241 ,8282685 ,1670935 17220269 23535029 23818625 21435182 25691471</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:19, 19 February 2015
- Description: nitrogen-regulated PII protein
| Gene name | nrgB |
| Synonyms | glnK |
| Essential | no |
| Product | nitrogen-regulated PII protein |
| Function | regulation of ammonium uptake |
| Gene expression levels in SubtiExpress: nrgB | |
| Interactions involving this protein in SubtInteract: NrgB | |
| Metabolic function and regulation of this protein in SubtiPathways: nrgB | |
| MW, pI | 12 kDa, 8.825 |
| Gene length, protein length | 348 bp, 116 aa |
| Immediate neighbours | nrgA, bcrC |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, glutamate metabolism, transcription factors and their control, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36520
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: P(II) protein family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane, via NrgA PubMed
Database entries
- UniProt: Q07428
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant: GP259 (cat), GP255 (nrgA-nrgB, cat), both available in Jörg Stülke's lab PubMed
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Jörg Stülke lab
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
Your additional remarks
References
Reviews
Cheng-Cai Zhang, Cong-Zhao Zhou
ATPase as a switch in P(II) signal transduction.
Proc Natl Acad Sci U S A: 2013, 110(32);12863-4
[PubMed:23847201]
[WorldCat.org]
[DOI]
(I p)
Karl Forchhammer
P(II) signal transducers: novel functional and structural insights.
Trends Microbiol: 2008, 16(2);65-72
[PubMed:18182294]
[WorldCat.org]
[DOI]
(P p)
T Arcondéguy, R Jack, M Merrick
P(II) signal transduction proteins, pivotal players in microbial nitrogen control.
Microbiol Mol Biol Rev: 2001, 65(1);80-105
[PubMed:11238986]
[WorldCat.org]
[DOI]
(P p)
G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet: 2000, 16(1);11-4
[PubMed:10637624]
[WorldCat.org]
[DOI]
(P p)
Original publications
