Difference between revisions of "YwlE"
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**''ywlE::tet'' available from [[Ulf Gerth]]'s lab | **''ywlE::tet'' available from [[Ulf Gerth]]'s lab | ||
**''ywlE::spec'' available from [[Ulf Gerth]]'s lab | **''ywlE::spec'' available from [[Ulf Gerth]]'s lab | ||
| − | **GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab | + | ** GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab |
| − | **GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s | + | ** GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s and [[Fabian Commichau]]'s labs {{PubMed|25610436}} |
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab | * '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab | ||
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=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
* [[Ulf Gerth]], Greifswald, Germany | * [[Ulf Gerth]], Greifswald, Germany | ||
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* [[Fabian Commichau]] Göttingen, Germany | * [[Fabian Commichau]] Göttingen, Germany | ||
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=References= | =References= | ||
| − | <pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 </pubmed> | + | <pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 25610436</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 09:06, 23 January 2015
- Description: protein arginine phosphatase
| Gene name | ywlE |
| Synonyms | ipc-31d |
| Essential | no |
| Product | protein arginine phosphatase |
| Function | dephosphorylation of McsB |
| Gene expression levels in SubtiExpress: ywlE | |
| MW, pI | 16 kDa, 6.681 |
| Gene length, protein length | 450 bp, 150 aa |
| Immediate neighbours | ywlF, ywlD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36930
Phenotypes of a mutant
Database entries
- BsubCyc: BSU36930
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein arginine phosphate + H2O = protein arginine + phosphate PubMed
- Protein family:
- Paralogous protein(s): YfkJ
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: weak oligomerization, results in inactivation of YwlE PubMed
Database entries
- BsubCyc: BSU36930
- Structure: 1ZGG
- UniProt: P39155
- KEGG entry: [2]
- E.C. number: 3.1.3.48
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- ywlE::aphA3 available from Ulf Gerth's lab
- ywlE::tet available from Ulf Gerth's lab
- ywlE::spec available from Ulf Gerth's lab
- GP1458 (ywlE::aphA3), available in Jörg Stülke's lab
- GP1459 (ywlE::tet), available in Jörg Stülke's and Fabian Commichau's labs PubMed
- Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody: available in Ulf Gerth's lab
Labs working on this gene/protein
- Ulf Gerth, Greifswald, Germany
- Fabian Commichau Göttingen, Germany
Your additional remarks
References
