Difference between revisions of "FliL"
| Line 43: | Line 43: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
{{SubtiWiki regulon|[[CodY regulon]]}}, | {{SubtiWiki regulon|[[CodY regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[DegU regulon]]}}, | ||
{{SubtiWiki regulon|[[SigD regulon]]}}, | {{SubtiWiki regulon|[[SigD regulon]]}}, | ||
{{SubtiWiki regulon|[[Spo0A regulon]]}} | {{SubtiWiki regulon|[[Spo0A regulon]]}} | ||
| Line 114: | Line 115: | ||
** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}} | ** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}} | ||
** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}} | ** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}} | ||
| − | + | ||
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 122: | Line 123: | ||
** [[Spo0A]]: transcription repression {{PubMed|14651647}} | ** [[Spo0A]]: transcription repression {{PubMed|14651647}} | ||
** [[CodY]]: transcription repression {{PubMed|12730172}} | ** [[CodY]]: transcription repression {{PubMed|12730172}} | ||
| + | ** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}} | ||
| + | ** [[DegU]]-P: transcription repression {{PubMed|24386445}} | ||
| + | ** [[DegU]]-P in complex with [[SwrA]]: transcription activation {{PubMed|24386445}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 145: | Line 149: | ||
=References= | =References= | ||
| − | <pubmed>14651647, 9657996,8157612,15175317 17850253 21821766</pubmed> | + | <pubmed>14651647, 9657996,8157612,15175317 17850253 21821766 24386445</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:43, 29 December 2014
- Description: flagellar protein required for flagellar formation , membrane protein
| Gene name | fliL |
| Synonyms | |
| Essential | no |
| Product | flagellar protein required for flagellar formation , membrane protein |
| Function | movement and chemotaxis |
| Gene expression levels in SubtiExpress: fliL | |
| MW, pI | 15 kDa, 5.175 |
| Gene length, protein length | 420 bp, 140 aa |
| Immediate neighbours | ylzI, fliM |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
motility and chemotaxis, membrane proteins
This gene is a member of the following regulons
CodY regulon, DegU regulon, SigD regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU16300
Phenotypes of a mutant
Database entries
- BsubCyc: BSU16300
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relexed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: fliL family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU16300
- Structure:
- UniProt: P23452
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio
The role of SwrA, DegU and P(D3) in fla/che expression in B. subtilis.
PLoS One: 2013, 8(12);e85065
[PubMed:24386445]
[WorldCat.org]
[DOI]
(I e)
Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766]
[WorldCat.org]
[DOI]
(I p)
Kazuo Kobayashi
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis.
Mol Microbiol: 2007, 66(2);395-409
[PubMed:17850253]
[WorldCat.org]
[DOI]
(P p)
H Werhane, P Lopez, M Mendel, M Zimmer, G W Ordal, L M Márquez-Magaña
The last gene of the fla/che operon in Bacillus subtilis, ylxL, is required for maximal sigmaD function.
J Bacteriol: 2004, 186(12);4025-9
[PubMed:15175317]
[WorldCat.org]
[DOI]
(P p)
Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647]
[WorldCat.org]
[DOI]
(P p)
W Estacio, S S Anna-Arriola, M Adedipe, L M Márquez-Magaña
Dual promoters are responsible for transcription initiation of the fla/che operon in Bacillus subtilis.
J Bacteriol: 1998, 180(14);3548-55
[PubMed:9657996]
[WorldCat.org]
[DOI]
(P p)
L M Márquez-Magaña, M J Chamberlin
Characterization of the sigD transcription unit of Bacillus subtilis.
J Bacteriol: 1994, 176(8);2427-34
[PubMed:8157612]
[WorldCat.org]
[DOI]
(P p)
