AraR

• Description: transcriptional repressor (GntR family, LacI family) of the arabinose utilization genes
  
  

Gene name	araR
Synonyms	araC, yvbS
Essential	no
Product	transcriptional repressor (GntR family, LacI family)
Function	regulation of arabinose utilization (araABDLMNPQ-abfA) and of the araE/araR genes
Gene expression levels in SubtiExpress: araR (araABDLMNPQ-abfA) and of the araE/araR genes
Metabolic function and regulation of this protein in SubtiPathways: AraR
MW, pI	43 kDa, 6.341
Gene length, protein length	1152 bp, 384 aa
Immediate neighbours	araE, yvbT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

AraR regulon

The gene

Basic information

• Locus tag: BSU33970

Database entries

• BsubCyc: BSU33970

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: GntR family: N-terminal DNA-binding domain, LacI family: C-terminal effector-binding domain

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal DNA-binding domain (GntR family)
  • C-terminal effector-binding domain (LacI family)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU33970

• Structure: 3TB6 (effector-binding domain) PubMed

• UniProt: P96711

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: araR PubMed

• Expression browser: araR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by arabinose (AraR) PubMed

• Regulatory mechanism:
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Isabel Lopes Correia, Irina Saraiva Franco, Isabel de Sá-Nogueira
Towards novel amino acid-base contacts in gene regulatory proteins: AraR--a case study.
PLoS One: 2014, 9(11);e111802
[PubMed:25364981] [WorldCat.org] [DOI] (I e)

Deepti Jain, Deepak T Nair
Spacing between core recognition motifs determines relative orientation of AraR monomers on bipartite operators.
Nucleic Acids Res: 2013, 41(1);639-47
[PubMed:23109551] [WorldCat.org] [DOI] (I p)

Kateřina Procházková, Kateřina Cermáková, Petr Pachl, Irena Sieglová, Milan Fábry, Zbyszek Otwinowski, Pavlína Rezáčová
Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2012, 68(Pt 2);176-85
[PubMed:22281747] [WorldCat.org] [DOI] (I p)

Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
Nucleic Acids Res: 2007, 35(14);4755-66
[PubMed:17617643] [WorldCat.org] [DOI] (I p)

Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Functional domains of the Bacillus subtilis transcription factor AraR and identification of amino acids important for nucleoprotein complex assembly and effector binding.
J Bacteriol: 2006, 188(8);3024-36
[PubMed:16585763] [WorldCat.org] [DOI] (P p)

Maria Paiva Raposo, José Manuel Inácio, Luís Jaime Mota, Isabel de Sá-Nogueira
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.
J Bacteriol: 2004, 186(5);1287-96
[PubMed:14973026] [WorldCat.org] [DOI] (P p)

L J Mota, L M Sarmento, I de Sá-Nogueira
Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping.
J Bacteriol: 2001, 183(14);4190-201
[PubMed:11418559] [WorldCat.org] [DOI] (P p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, L J Mota
Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene.
J Bacteriol: 1997, 179(5);1598-608
[PubMed:9045819] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, H Paveia, H de Lencastre
Isolation of constitutive mutants for L-arabinose utilization in Bacillus subtilis.
J Bacteriol: 1988, 170(6);2855-7
[PubMed:3131313] [WorldCat.org] [DOI] (P p)