Difference between revisions of "Scr"

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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU_MISC_RNA_2&redirect=T BSU_misc_RNA_2]
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU_MISC_RNA_2&redirect=T BSU_misc_RNA_2]
  
* '''Structure''':  
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* '''Structure''': [http://www.rcsb.org/pdb/explore.do?structureId=4WFL 4WFL] {{PubMed|25270875}}
  
 
=== Additional information===
 
=== Additional information===
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<pubmed> 25212246 </pubmed>
 
<pubmed> 25212246 </pubmed>
 
== Original publications ==
 
== Original publications ==
<pubmed>9346318,2468993,17576666, 1372600,7506707 1383945 7538759 10224127 9677377 8662730 10222262</pubmed>
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<pubmed>9346318,2468993,17576666, 1372600,7506707 1383945 7538759 10224127 9677377 8662730 10222262 25270875 </pubmed>
  
 
[[Category:small RNA]]
 
[[Category:small RNA]]

Revision as of 15:22, 4 October 2014

  • Description: 4.5 S RNA

Gene name scr
Synonyms
Essential yes
Product small cytoplasmic RNA (scRNA, 4.5S RNA),

signal recognition particle-like (SRP) component

Function presecretory protein translocation
Interactions involving this RNA in SubtInteract: Scr
Immediate neighbours yaaJ, dnaX
Gene sequence (+200bp) Protein sequence
Genetic context
Scr context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

protein secretion, essential genes, ncRNA

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

  • the gene is essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]
  • Structure:

Additional information

The RNA

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:

Extended information on the RNA

  • Kinetic information

Database entries

Additional information

Expression and regulation

  • Sigma factor: SigA (both promoters)
  • Regulation:
  • Regulatory mechanism:
  • Additional information: transcribed as 354 nt precursor, cleaved at the 5' end by RNase III and at the 3' site by RNase J1 to yield a 275 nt molecule, final trimming to 271 nt sc RNA by RNase PH PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

Your additional remarks

References

Reviews

Zhen Kang, Sen Yang, Guocheng Du, Jian Chen
Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species.
J Ind Microbiol Biotechnol: 2014, 41(11);1599-607
[PubMed:25212246] [WorldCat.org] [DOI] (I p)

Original publications

Georg Kempf, Klemens Wild, Irmgard Sinning
Structure of the complete bacterial SRP Alu domain.
Nucleic Acids Res: 2014, 42(19);12284-94
[PubMed:25270875] [WorldCat.org] [DOI] (I p)

Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666] [WorldCat.org] [DOI] (I p)

K Nakamura, S Yahagi, T Yamazaki, K Yamane
Bacillus subtilis histone-like protein, HBsu, is an integral component of a SRP-like particle that can bind the Alu domain of small cytoplasmic RNA.
J Biol Chem: 1999, 274(19);13569-76
[PubMed:10224127] [WorldCat.org] [DOI] (P p)

T Yamazaki, S Yahagi, K Nakamura, K Yamane
Depletion of Bacillus subtilis histone-like protein, HBsu, causes defective protein translocation and induces upregulation of small cytoplasmic RNA.
Biochem Biophys Res Commun: 1999, 258(1);211-4
[PubMed:10222262] [WorldCat.org] [DOI] (P p)

A Oguro, H Kakeshita, K Nakamura, K Yamane, W Wang, D H Bechhofer
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
J Biol Chem: 1998, 273(31);19542-7
[PubMed:9677377] [WorldCat.org] [DOI] (P p)

H Takamatsu, K Bunai, T Horinaka, A Oguro, K Nakamura, K Watabe, K Yamane
Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle.
Eur J Biochem: 1997, 248(2);575-82
[PubMed:9346318] [WorldCat.org] [DOI] (P p)

K Kurita, K Honda, S Suzuma, H Takamatsu, K Nakamura, K Yamane
Identification of a region of Bacillus subtilis Ffh, a homologue of mammalian SRP54 protein, that is essential for binding to small cytoplasmic RNA.
J Biol Chem: 1996, 271(22);13140-6
[PubMed:8662730] [WorldCat.org] [DOI] (P p)

T Shibata, Y Nakamura, K Nakamura, K Yamane
Depletion of small cytoplasmic RNA confers fusidic-acid resistance on Bacillus subtilis.
Biochem Biophys Res Commun: 1995, 210(2);317-23
[PubMed:7538759] [WorldCat.org] [DOI] (P p)

M Nishiguchi, K Honda, R Amikura, K Nakamura, K Yamane
Structural requirements of Bacillus subtilis small cytoplasmic RNA for cell growth, sporulation, and extracellular enzyme production.
J Bacteriol: 1994, 176(1);157-65
[PubMed:7506707] [WorldCat.org] [DOI] (P p)

K Nakamura, M Minemura, M Nishiguchi, K Honda, A Nakamura, K Yamane
Conserved residues and secondary structure found in small cytoplasmic RNAs from thirteen Bacillus species.
Nucleic Acids Res: 1992, 20(19);5227-8
[PubMed:1383945] [WorldCat.org] [DOI] (P p)

K Nakamura, Y Imai, A Nakamura, K Yamane
Small cytoplasmic RNA of Bacillus subtilis: functional relationship with human signal recognition particle 7S RNA and Escherichia coli 4.5S RNA.
J Bacteriol: 1992, 174(7);2185-92
[PubMed:1372600] [WorldCat.org] [DOI] (P p)

J C Struck, R K Hartmann, H Y Toschka, V A Erdmann
Transcription and processing of Bacillus subtilis small cytoplasmic RNA.
Mol Gen Genet: 1989, 215(3);478-82
[PubMed:2468993] [WorldCat.org] [DOI] (P p)