Difference between revisions of "SpsI"
| Line 42: | Line 42: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[GerE regulon]]}}, | ||
{{SubtiWiki regulon|[[SigK regulon]]}} | {{SubtiWiki regulon|[[SigK regulon]]}} | ||
| Line 107: | Line 108: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsI_3885239_3885979_-1 spsI] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spsI_3885239_3885979_-1 spsI] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigK]] {{PubMed|15383836}} | + | * '''[[Sigma factor]]:''' [[SigK]] {{PubMed|15383836}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** expressed late during sporulation in the mother cell ([[SigK]]) {{PubMed|15383836}} | + | ** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed|25239894,15383836}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[GerE]]: transcription activation {{PubMed|25239894,15383836}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 136: | Line 138: | ||
=References= | =References= | ||
| − | <pubmed>9353933 15383836 12884008 21821766 22960854</pubmed> | + | <pubmed>9353933 15383836 12884008 21821766 22960854 25239894</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 11:01, 23 September 2014
- Description: glucose-1-phosphate thymidylyltransferase, spore coat polysaccharide synthesis
| Gene name | spsI |
| Synonyms | ipa-71d |
| Essential | no |
| Product | glucose-1-phosphate thymidylyltransferase |
| Function | rhamnose biosynthesis, spore coat polysaccharide synthesis |
| Gene expression levels in SubtiExpress: spsI | |
| MW, pI | 27 kDa, 5.385 |
| Gene length, protein length | 738 bp, 246 aa |
| Immediate neighbours | spsJ, spsG |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
miscellaneous metabolic pathways, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37840
Phenotypes of a mutant
Database entries
- BsubCyc: BSU37840
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- alpha-D-glucose-1-phosphate + dTTP --> dTDP-glucose PubMed
- Protein family: glucose-1-phosphate thymidylyltransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU37840
- Structure:
- UniProt: P39629
- KEGG entry: [3]
- E.C. number: 2.7.7.24
Additional information
Expression and regulation
- Additional information:
- the mRNA is very stable (half-life > 15 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Giuseppina Cangiano, Teja Sirec, Cristina Panarella, Rachele Isticato, Loredana Baccigalupi, Maurilio De Felice, Ezio Ricca
The sps Gene Products Affect the Germination, Hydrophobicity, and Protein Adsorption of Bacillus subtilis Spores.
Appl Environ Microbiol: 2014, 80(23);7293-302
[PubMed:25239894]
[WorldCat.org]
[DOI]
(I p)
Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854]
[WorldCat.org]
[DOI]
(I p)
Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766]
[WorldCat.org]
[DOI]
(I p)
Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836]
[WorldCat.org]
[DOI]
(I p)
G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008]
[WorldCat.org]
[DOI]
(P p)
E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933]
[WorldCat.org]
[DOI]
(P p)
