PhoD

• Description: phosphodiesterase/alkaline phosphatase
  
  

Gene name	phoD
Synonyms	ycbS
Essential	no
Product	phosphodiesterase/alkaline phosphatase
Function	aquisition of phosphate upon phosphoate starvation
Gene expression levels in SubtiExpress: phoD
Interactions involving this protein in SubtInteract: PhoD
Metabolic function and regulation of this protein in SubtiPathways: PhoD
MW, pI	62 kDa, 8.394
Gene length, protein length	1668 bp, 556 aa
Immediate neighbours	yczK, tatAD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphate metabolism

This gene is a member of the following regulons

PhoP regulon

The gene

Basic information

• Locus tag: BSU02620

Database entries

• BsubCyc: BSU02620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A phosphate monoester + H₂O = an alcohol + phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • one Fe³⁺ and two Ca²⁺ PubMed

• Effectors of protein activity:

• Interactions:
  • TatAD-PhoD PubMed

• Localization: extracellular (signal peptide) PubMed, secreted by the TatAD-TatCD complex PubMed

Database entries

• BsubCyc: BSU02620

• Structure: 2YEQ PubMed

• UniProt: P42251

• KEGG entry: [3]

• E.C. number: 3.1.3.1

Expression and regulation

• Operon: phoD-tatAD-tatCD PubMed

• Expression browser: phoD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:
  • expression of the operon depends on functional HemAT and CsbC PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Oscar Kuipers, University of Groningen, The Netherlands Homepage

References

Reviews

Vivianne J Goosens, Carmine G Monteferrante, Jan Maarten van Dijl
The Tat system of Gram-positive bacteria.
Biochim Biophys Acta: 2014, 1843(8);1698-706
[PubMed:24140208] [WorldCat.org] [DOI] (P p)