Hmp

• Description: flavohemoglobin, involved in resistance to nitric oxide (NO), essential for long-term survival under anaerobic conditions
  
  

Gene name	hmp
Synonyms	ykiA
Essential	no
Product	flavohemoglobin
Function	resistance to NO
Gene expression levels in SubtiExpress: hmp
Metabolic function and regulation of this protein in SubtiPathways: hmp
MW, pI	44 kDa, 5.705
Gene length, protein length	1197 bp, 399 aa
Immediate neighbours	ykhA, ykzH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

NsrR regulon, ResD regulon

The gene

Basic information

• Locus tag: BSU13040

Database entries

• BsubCyc: BSU13040

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 NO + 2 O₂ + NAD(P)H = 2 NO₃^- + NAD(P)⁺ (according to Swiss-Prot)

• Protein family: FAD-binding FR-type domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU13040

• Structure:

• UniProt: P49852

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • hmp PubMed
  • hmp-ykjA PubMed

• Expression browser: hmp PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed
  • induced by nitric oxide under anaerobic conditions (NsrR) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed
  • NsrR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 27 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 346 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: