AdaA

• Description: trigger enzyme: methylphosphotriester-DNA alkyltransferase/ transcriptional activator of the adaA-adaB operon
  
  

Gene name	adaA
Synonyms
Essential	no
Product	trigger enzyme: methylphosphotriester-DNA alkyltransferase/ transcriptional activator of the adaAB operon
Function	adaptive response to alkylative DNA damage
Gene expression levels in SubtiExpress: adaA
MW, pI	24 kDa, 9.185
Gene length, protein length	633 bp, 211 aa
Immediate neighbours	alkA, adaB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, transcription factors and their control, trigger enzyme, prophage 1

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

• Locus tag: BSU01810

Database entries

• BsubCyc: BSU01810

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU01810

• Structure:

• UniProt: P19219

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: adaA-adaB PubMed

• Expression browser: adaA PubMed

• Sigma factor: SigA PubMed

• Regulation: positive control by AdaA PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Damian Mielecki, Elżbieta Grzesiuk
Ada response - a strategy for repair of alkylated DNA in bacteria.
FEMS Microbiol Lett: 2014, 355(1);1-11
[PubMed:24810496] [WorldCat.org] [DOI] (I p)

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)

F Morohoshi, K Hayashi, N Munakata
Bacillus subtilis ada operon encodes two DNA alkyltransferases.
Nucleic Acids Res: 1990, 18(18);5473-80
[PubMed:2120677] [WorldCat.org] [DOI] (P p)