RecU

• Description: Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU
  
  

Gene name	recU
Synonyms	recG, prfA, yppB, jopB
Essential	no
Product	Holliday junction resolvase
Function	DNA repair/ recombination and chromosome segregation
Gene expression levels in SubtiExpress: recU
Interactions involving this protein in SubtInteract: RecU
MW, pI	23 kDa, 9.511
Gene length, protein length	618 bp, 206 aa
Immediate neighbours	yppC, ponA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU22310

Phenotypes of a mutant

• the inactivations of recU and ruvA-ruvB are synthetically lethal PubMed

Database entries

• BsubCyc: BSU22310

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds Holliday junctions with high affinity PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • RecU-mediated Holliday junction resolution is stimulated by RuvB-ATP PubMed

• Interactions:
  • form dimers PubMed
  • RecA-RecU PubMed
  • RecU-RuvB PubMed
  • formation of a ternary complex RecU-RuvB-Holliday junction DNA PubMed

• Localization:

Database entries

• BsubCyc: BSU22310

• Structure: 1ZP7

• UniProt: P39792

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recU-ponA PubMed

• Expression browser: recU PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP891 (recU::cat), available in Jörg Stülke's lab
  • 1A895 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

S C West
Processing of recombination intermediates by the RuvABC proteins.
Annu Rev Genet: 1997, 31;213-44
[PubMed:9442895] [WorldCat.org] [DOI] (P p)

Original publications

Cristina Cañas, Yuki Suzuki, Chiara Marchisone, Begoña Carrasco, Verónica Freire-Benéitez, Kunio Takeyasu, Juan C Alonso, Silvia Ayora
Interaction of branch migration translocases with the Holliday junction-resolving enzyme and their implications in Holliday junction resolution.
J Biol Chem: 2014, 289(25);17634-46
[PubMed:24770420] [WorldCat.org] [DOI] (I p)

Cristina Cañas, Begoña Carrasco, Esther García-Tirado, John B Rafferty, Juan C Alonso, Silvia Ayora
The stalk region of the RecU resolvase is essential for Holliday junction recognition and distortion.
J Mol Biol: 2011, 410(1);39-49
[PubMed:21600217] [WorldCat.org] [DOI] (I p)

Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann
Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells.
PLoS Genet: 2009, 5(9);e1000630
[PubMed:19730681] [WorldCat.org] [DOI] (I p)

Begoña Carrasco, Cristina Cañas, Gary J Sharples, Juan C Alonso, Silvia Ayora
The N-terminal region of the RecU holliday junction resolvase is essential for homologous recombination.
J Mol Biol: 2009, 390(1);1-9
[PubMed:19422832] [WorldCat.org] [DOI] (I p)

Cristina Cañas, Begoña Carrasco, Silvia Ayora, Juan C Alonso
The RecU Holliday junction resolvase acts at early stages of homologous recombination.
Nucleic Acids Res: 2008, 36(16);5242-9
[PubMed:18684995] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Natalie McGregor, Sylvia Ayora, Svetlana Sedelnikova, Begona Carrasco, Juan C Alonso, Paul Thaw, John Rafferty
The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage.
Structure: 2005, 13(9);1341-51
[PubMed:16154091] [WorldCat.org] [DOI] (P p)

Begoña Carrasco, Silvia Ayora, Rudi Lurz, Juan C Alonso
Bacillus subtilis RecU Holliday-junction resolvase modulates RecA activities.
Nucleic Acids Res: 2005, 33(12);3942-52
[PubMed:16024744] [WorldCat.org] [DOI] (I e)

Humberto Sanchez, Dawit Kidane, Patricia Reed, Fiona A Curtis, M Castillo Cozar, Peter L Graumann, Gary J Sharples, Juan C Alonso
The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis.
Genetics: 2005, 171(3);873-83
[PubMed:16020779] [WorldCat.org] [DOI] (P p)

Begoña Carrasco, M Castillo Cozar, Rudi Lurz, Juan C Alonso, Silvia Ayora
Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation.
J Bacteriol: 2004, 186(17);5557-66
[PubMed:15317759] [WorldCat.org] [DOI] (P p)

B Carrasco, S Fernández, K Asai, N Ogasawara, J C Alonso
Effect of the recU suppressors sms and subA on DNA repair and homologous recombination in Bacillus subtilis.
Mol Genet Genomics: 2002, 266(5);899-906
[PubMed:11810266] [WorldCat.org] [DOI] (P p)

S Fernández, A Sorokin, J C Alonso
Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination.
J Bacteriol: 1998, 180(13);3405-9
[PubMed:9642195] [WorldCat.org] [DOI] (P p)

D L Popham, P Setlow
Cloning, nucleotide sequence, and mutagenesis of the Bacillus subtilis ponA operon, which codes for penicillin-binding protein (PBP) 1 and a PBP-related factor.
J Bacteriol: 1995, 177(2);326-35
[PubMed:7814321] [WorldCat.org] [DOI] (P p)

RecU in other organisms

Ana R Pereira, Patricia Reed, Helena Veiga, Mariana G Pinho
The Holliday junction resolvase RecU is required for chromosome segregation and DNA damage repair in Staphylococcus aureus.
BMC Microbiol: 2013, 13;18
[PubMed:23356868] [WorldCat.org] [DOI] (I e)