sufB

168

FeS scaffold, synthesis of Fe-S-clusters

Locus: BSU_32670

Molecular weight: 52.56 kDa

Isoelectric point: 4.96

Protein length: 465 aa

Gene length: 1398 bp

Function: synthesis of iron-sulfur clusters

Product: FeS scaffold

Essential: yes

Synonyms: sufB, yurU

Genomic Context

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List of homologs in different organisms, belongs to COG0719 (Galperin et al., 2021)

Gene

Coordinates: 3,355,593 3,356,990

Phenotypes of a mutant

• essential PubMed
• the mutant is viable and Fe-S clusters are dispensable if IspG and IspH from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate PubMed

The protein

Catalyzed reaction/ biological activity

• synthesis of Fe-S-clusters (the SufB-SufC-SufD complex) PubMed

Protein family

• UPF0051 (ycf24) family (with SufD, according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 5AWF (PDB) (from E. coli, the SufB-SufC-SufD complex, 41% identity) PubMed
• O32162 (AlphaFold)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: sufC-sufD-sufS-sufU-sufB

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B583 (yurU::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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Original Publications

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