ResD

• Description: two-component response regulator, regulation of aerobic and anaerobic respiration
  
  

Gene name	resD
Synonyms	ypxD
Essential	no
Product	two-component response regulator
Function	regulation of aerobic and anaerobic respiration respiration
Gene expression levels in SubtiExpress: resD
Interactions involving this protein in SubtInteract: ResD
Metabolic function and regulation of this protein in SubtiPathways: resD
MW, pI	27 kDa, 5.631
Gene length, protein length	720 bp, 240 aa
Immediate neighbours	resE, resC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

regulators of electron transport, transcription factors and their control, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, ResD regulon

The gene

Basic information

• Locus tag: BSU23120

Database entries

• BsubCyc: BSU23120

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpR family of two-component response regulators

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by ResE on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions:
  • ResD interacts with the RNA polymerase PubMed
  • ResE-ResD

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU23120

• Structure:

• UniProt: P35163

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • resA-resB-resC-resD-resE PubMed
  • resD-resE PubMed

• Expression browser: resD PubMed

• Sigma factor:
  • resA: SigA PubMed
  • resD: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed
  • PhoP: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 306 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3258 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 909 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 838 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 969 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Reviews

Elisabeth Härtig, Dieter Jahn
Regulation of the anaerobic metabolism in Bacillus subtilis.
Adv Microb Physiol: 2012, 61;195-216
[PubMed:23046954] [WorldCat.org] [DOI] (I p)