PpaC

• Description: inorganic pyrophosphatase
  
  

Gene name	ppaC
Synonyms	yybQ
Essential	yes PubMed
Product	inorganic pyrophosphatase
Function	recovery of phosphate ions from pyrophosphate
Gene expression levels in SubtiExpress: ppaC
MW, pI	33 kDa, 4.513
Gene length, protein length	927 bp, 309 aa
Immediate neighbours	hypR, yybP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

phosphate metabolism, essential genes, most abundant proteins

The gene

Basic information

• Locus tag: BSU40550

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU40550

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Diphosphate + H₂O = 2 phosphate (according to Swiss-Prot)

• Protein family: PPase class C family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • S-cysteinylation after diamide stress (C158) PubMed
  • Cys158 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU40550

• Structure: 2HAW (complex with substrate analogue p-nitrophosphate), 1WPM

• UniProt: P37487

• KEGG entry: [3]

• E.C. number: 3.6.1.1

Expression and regulation

• Operon: ppaC PubMed

• Expression browser: ppaC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 4150 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 10163 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 479 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 509 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 611 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: