Difference between revisions of "GuaA"
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 2612 {{PubMed|24696501}} | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 2612 {{PubMed|24696501}} | ||
** number of protein molecules per cell (complex medium with amino acids, without glucose): 12454 {{PubMed|24696501}} | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 12454 {{PubMed|24696501}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4578 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 5511 {{PubMed|21395229}} | ||
| + | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 7521 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
| − | |||
* '''Mutant:''' | * '''Mutant:''' | ||
Revision as of 14:07, 17 April 2014
- Description: GMP synthase (glutamine-hydrolysing)
| Gene name | guaA |
| Synonyms | guaB |
| Essential | no |
| Product | GMP synthetase (glutamine-hydrolysing) |
| Function | biosynthesis of GMP |
| Gene expression levels in SubtiExpress: guaA | |
| Metabolic function and regulation of this protein in SubtiPathways: guaA | |
| MW, pI | 57 kDa, 4.747 |
| Gene length, protein length | 1539 bp, 513 aa |
| Immediate neighbours | yebA, pbuG |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06360
Phenotypes of a mutant
Database entries
- BsubCyc: BSU06360
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- the enzyme is inhibited by guanosine tetraphosphate (stringent response) PubMed
Database entries
- BsubCyc: BSU06360
- Structure: 2YWB (from Thermus thermophilus hb8, 45% identity, 57% similarity)
- UniProt: P29727
- KEGG entry: [3]
- E.C. number: 6.3.5.2
Additional information
Expression and regulation
- Operon: guaA PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 2612 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 12454 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4578 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 5511 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 7521 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
