RnjB

• Description: RNase J2
  
  

Gene name	rnjB
Synonyms	ymfA
Essential	no
Product	RNase J2
Function	RNA processing and degradation
Gene expression levels in SubtiExpress: rnjB
Interactions involving this protein in SubtInteract: RNase J2
MW, pI	56 kDa, 9.18
Gene length, protein length	1545 bp, 515 aa
Immediate neighbours	dapA, tepA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases

The gene

Basic information

• Locus tag: BSU16780

Database entries

• BsubCyc: BSU16780

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: endoribonuclease, involved in processing of thrS mRNA

• Protein family: RNase J subfamily (according to Swiss-Prot)

• Paralogous protein(s): RnjA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • associated to the RNA degradosome PubMed
  • RnjA-RnjB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16780

• Structure: 3ZQ4 (RNase J1) PubMed

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: rnjB PubMed

• Expression browser: rnjB PubMed

• Sigma factor: SigA PubMed

• Regulation: constitutive expression PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1201 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3208 PubMed

Biological materials

• Mutant: GP45 (spc), GP1113 (miniTn10 spc), both available in Stülke lab

• Expression vector:

• lacZ fusion: pGP419 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP1001 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

Labs working on this gene/protein

Harald Putzer, IBPC Paris, France Homepage