Difference between revisions of "TlpB"

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* '''Additional information:'''  
 
* '''Additional information:'''  
 
** in minimal medium, TlpB is present with 2,500 +/- 740 molecules per cell {{PubMed|21515776}}
 
** in minimal medium, TlpB is present with 2,500 +/- 740 molecules per cell {{PubMed|21515776}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 36 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 77 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:36, 17 April 2014

  • Description: membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein

Gene name tlpB
Synonyms
Essential no
Product methyl-accepting chemotaxis protein
Function control of chemotaxis
Gene expression levels in SubtiExpress: tlpB
MW, pI 71 kDa, 4.733
Gene length, protein length 1986 bp, 662 aa
Immediate neighbours yuxG, mcpA
Sequences Protein DNA DNA_with_flanks
Genetic context
TlpB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TlpB expression.png
























Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU31230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, TlpB is present with 2,500 +/- 740 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 36 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 77 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776] [WorldCat.org] [DOI] (I p)

D W Hanlon, G W Ordal
Cloning and characterization of genes encoding methyl-accepting chemotaxis proteins in Bacillus subtilis.
J Biol Chem: 1994, 269(19);14038-46
[PubMed:8188684] [WorldCat.org] (P p)