PepF

• Description: oligoendopeptidase, inhibits sporulation upon overexpression
  
  

Gene name	pepF
Synonyms	yjbG
Essential	no
Product	oligoendopeptidase
Function	protein degradation
Gene expression levels in SubtiExpress: pepF
MW, pI	69 kDa, 5.131
Gene length, protein length	1827 bp, 609 aa
Immediate neighbours	coiA, yizD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

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The gene

Basic information

• Locus tag: BSU11540

Database entries

• BsubCyc: BSU11540

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase M3B family (according to Swiss-Prot)

• Paralogous protein(s): YusX

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU11540

• Structure:

• UniProt: O31605

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: pepF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2180 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2347 PubMed

Biological materials

• Mutant:
  • 1A823 ( pepF::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kyoko Kanamaru, Sophie Stephenson, Marta Perego
Overexpression of the PepF oligopeptidase inhibits sporulation initiation in Bacillus subtilis.
J Bacteriol: 2002, 184(1);43-50
[PubMed:11741842] [WorldCat.org] [DOI] (P p)