Difference between revisions of "CwlO"

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** the leader mRNA is subject to cleavage by [[rny|RNase Y]], this results in limited expression of ''[[cwlO]]'' {{PubMed|24163346}}
 
** the leader mRNA is subject to cleavage by [[rny|RNase Y]], this results in limited expression of ''[[cwlO]]'' {{PubMed|24163346}}
 
** there is a [[SigB]]-dependent anti-sense transcript which is expressed in the stationary phase {{PubMed|24163346}}
 
** there is a [[SigB]]-dependent anti-sense transcript which is expressed in the stationary phase {{PubMed|24163346}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 207 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 200 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:27, 17 April 2014

  • Description: D,L-endopeptidase-type autolysin, primary autolytic pathway for cell elongation

Gene name cwlO
Synonyms yzkA, yvcE
Essential no
Product endopeptidase-type autolysin
Function cell wall synthesis, cell elongation
Gene expression levels in SubtiExpress: cwlO
Interactions involving this protein in SubtInteract: CwlO
MW, pI 50 kDa, 5.326
Gene length, protein length 1419 bp, 473 aa
Immediate neighbours trxB, yvcD
Sequences Protein DNA DNA_with_flanks
Genetic context
YvcE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CwlO expression.png















Categories containing this gene/protein

cell wall synthesis, cell wall degradation/ turnover

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

  • Locus tag: BSU34800

Phenotypes of a mutant

  • a cwlO lytE mutant is not viable PubMed
  • shorter, fatter cells, this can be rescued by addition of Mg(2+) PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase C40 family (according to Swiss-Prot)
  • Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Localization:
    • extracellular (signal peptide) PubMed
    • localizes to the outer lateral sidewall of the cell (via the N-terminal domain) PubMed
    • cell membrane in a FtsX-dependent manner PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • expressed during exponential growth (WalR) PubMed
  • Regulatory mechanism:
  • Additional information:
    • The mRNA has a long 5' leader region (187 N). This may indicate RNA-based regulation PubMed
    • the leader mRNA is subject to cleavage by RNase Y, this results in limited expression of cwlO PubMed
    • there is a SigB-dependent anti-sense transcript which is expressed in the stationary phase PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 207 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 200 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications