MetE

• Description: methionine synthase
  
  

Gene name	metE
Synonyms	metC
Essential	no
Product	methionine synthase
Function	biosynthesis of methionine
Gene expression levels in SubtiExpress: metE
Interactions involving this protein in SubtInteract: MetE
Metabolic function and regulation of this protein in SubtiPathways: metE
MW, pI	86 kDa, 4.839
Gene length, protein length	2286 bp, 762 aa
Immediate neighbours	guaD, ispA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13180

Database entries

• BsubCyc: BSU13180

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine (according to Swiss-Prot)

• Protein family: vitamin-B12 independent methionine synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on ser/ thr/ tyr PubMed, S-cysteinylation after diamide stress (C719) PubMed
  • Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
  • MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • BrxA-MetE, to de-bacillithiolate S-bacillithiolated MetE PubMed
  • BrxB-MetE, to de-bacillithiolate S-bacillithiolated MetE PubMed

• Localization:

Database entries

• BsubCyc: BSU13180

• Structure: 1T7L (from Thermotoga maritima, 44% identity, 61% similarity) PubMed

• UniProt: P80877

• KEGG entry: [2]

• E.C. number: 2.1.1.14

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: metE

• Expression browser: metE PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed by methionine (about 1000-fold)(S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:
  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 10685 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1682 PubMed

Biological materials

• Mutant:
  • 1A607 ( metE::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: