QdoI

• Description: Fe-containing quercetin 2,3-dioxygenase
  
  

Gene name	qdoI
Synonyms	yxaG
Essential	no
Product	Fe-containing quercetin 2,3-dioxygenase
Function	resistance to plant product quercetin
Gene expression levels in SubtiExpress: qdoI
MW, pI	37,4 kDa, 5.562
Gene length, protein length	1011 bp, 337 aa
Immediate neighbours	yxaH, qdoR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

LmrA regulon, QdoR regulon

The gene

Basic information

• Locus tag: BSU39980

Database entries

• BsubCyc: BSU39980

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: oxidation of the flavonol quercetin with dioxygen, cleaving the central heterocyclic ring and releasing CO

• Protein family:bicupin family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mn(II) (preferred), but also active with Fe(II) snd Co(II) PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU39980

• Structure: 2H0V

• UniProt: P42106

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: qdoI-yxaH PubMed

• Expression browser: qdoI PubMed

• Sigma factor:

• Regulation:
  • induced by flavonoids such as quercetin (QdoR, LmrA) PubMed

• Regulatory mechanism:
  • LmrA: transcription repression PubMed
  • QdoR: transcription repression PubMed

• Additional information:
  • major regulator: QdoR PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

J B Broderick
Catechol dioxygenases.
Essays Biochem: 1999, 34;173-89
[PubMed:10730195] [WorldCat.org] [DOI] (P p)

T D Bugg, J Sanvoisin, E L Spence
Exploring the catalytic mechanism of the extradiol catechol dioxygenases.
Biochem Soc Trans: 1997, 25(1);81-5
[PubMed:9056848] [WorldCat.org] [DOI] (P p)