Difference between revisions of "YqjM"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU23820&redirect=T BSU23820] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqjM.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqjM.html] | ||
| Line 87: | Line 88: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU23820&redirect=T BSU23820] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Z41 1Z41] (oxidized form), [http://www.rcsb.org/pdb/explore.do?structureId=1Z48 1Z48] (reduced form) | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Z41 1Z41] (oxidized form), [http://www.rcsb.org/pdb/explore.do?structureId=1Z48 1Z48] (reduced form) | ||
Revision as of 14:09, 2 April 2014
- Description: NADPH-dependent flavin oxidoreductase
| Gene name | yqjM |
| Synonyms | |
| Essential | no |
| Product | NADPH-dependent flavin oxidoreductase |
| Function | reduction of double bonds of nonsaturated aldehydes and
ketones, may be involved in detoxification |
| Gene expression levels in SubtiExpress: yqjM | |
| MW, pI | 37 kDa, 5.137 |
| Gene length, protein length | 1014 bp, 338 aa |
| Immediate neighbours | yqjN, yqjL |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
electron transport/ other, resistance against oxidative and electrophile stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23820
Phenotypes of a mutant
Database entries
- BsubCyc: BSU23820
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: NADPH + acceptor = NADP+ + reduced acceptor (according to Swiss-Prot)
- Protein family: NamA subfamily (according to Swiss-Prot)
- Paralogous protein(s): YqiG
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- BsubCyc: BSU23820
- UniProt: P54550
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: yqjM (according to DBTBS)
- Regulation:
- induced in the presence of H(2)O(2) PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Karina Kitzing, Teresa B Fitzpatrick, Corinna Wilken, Justyna Sawa, Gleb P Bourenkov, Peter Macheroux, Tim Clausen
The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.
J Biol Chem: 2005, 280(30);27904-13
[PubMed:15890652]
[WorldCat.org]
[DOI]
(P p)
Teresa B Fitzpatrick, Sigrid Auweter, Karina Kitzing, Tim Clausen, Nikolaus Amrhein, Peter Macheroux
Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.
Protein Expr Purif: 2004, 36(2);280-91
[PubMed:15249051]
[WorldCat.org]
[DOI]
(P p)
Teresa B Fitzpatrick, Nikolaus Amrhein, Peter Macheroux
Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response.
J Biol Chem: 2003, 278(22);19891-7
[PubMed:12660247]
[WorldCat.org]
[DOI]
(P p)
