BshB2

• Description: N-acetylglucosamine-malate deacetylase, minor enzyme involved in bacillithiol synthesis, may act as amidase for the processing of BSH conjugates
  
  

Gene name	bshB2
Synonyms	yojG
Essential	no
Product	N-acetylglucosamin-malate deacetylase
Function	biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshB2
MW, pI	16 kDa, 4.511
Gene length, protein length	426 bp, 142 aa
Immediate neighbours	rsbRC, yojF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU19460

Database entries

• BsubCyc: BSU19460

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): BshB1

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU19460

• Structure:

• UniProt: O31857

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • yojE-yoyC-yojF-bshB2 (minor transcript) PubMed
  • yoyC-yojF-bshB2 (major transcript) PubMed

• Expression browser: bshB2 PubMed

• Sigma factor:
  • yojE: SigA PubMed
  • yoyC: SigA PubMed

• Regulation:
  • yoyC: induced by diamide stess (thiol depletion) (Spx) PubMed

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant: bshB2 null mutant and bshB1 / bshB2 double null mutant available in John Helmann lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: