InfB

• Description: translation initiation factor IF-2
  
  

Gene name	infB
Synonyms
Essential	yes PubMed
Product	initiation factor IF-2
Function	translation
Gene expression levels in SubtiExpress: infB
MW, pI	78 kDa, 5.191
Gene length, protein length	2148 bp, 716 aa
Immediate neighbours	ylxQ, ylxP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU16630

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU16630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Initiation of translation

• Protein family: IF-2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: Nucleotide-binding domain that competitively binds GTP or ppGpp PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity: GTP and ppGpp PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16630

• Structure: 1D1N (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), 1Z9B (C1-subdomain, Geobacillus stearothermophilus)

• UniProt: P17889

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylxS-nusA-ylxR-ylxQ-infB-ylxP-rbfA PubMed

• Expression browser: infB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • Interaction with ppGpp interferes with IF2-dependent initiation complex formation, severely inhibits initiation dipeptide formation, and blocks the initiation step of translation.PubMed

• Regulatory mechanism:

• Additional information: Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pohl Milon, Eugene Tischenko, Jerneja Tomsic, Enrico Caserta, Gert Folkers, Anna La Teana, Marina V Rodnina, Cynthia L Pon, Rolf Boelens, Claudio O Gualerzi
The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor.
Proc Natl Acad Sci U S A: 2006, 103(38);13962-7
[PubMed:16968770] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

K Shazand, J Tucker, M Grunberg-Manago, J C Rabinowitz, T Leighton
Similar organization of the nusA-infB operon in Bacillus subtilis and Escherichia coli.
J Bacteriol: 1993, 175(10);2880-7
[PubMed:8491709] [WorldCat.org] [DOI] (P p)