Difference between revisions of "MgtE"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13300&redirect=T BSU13300] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13300&redirect=T BSU13300] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2QBZ 2QBZ] (the mgtE riboswitch) | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2QBZ 2QBZ] (the mgtE riboswitch) | ||
Revision as of 13:31, 2 April 2014
- Description: primary magnesium transporter
| Gene name | mgtE |
| Synonyms | ykoK |
| Essential | no |
| Product | primary magnesium transporter |
| Function | magnesium uptake |
| Gene expression levels in SubtiExpress: mgtE | |
| MW, pI | 50 kDa, 4.609 |
| Gene length, protein length | 1353 bp, 451 aa |
| Immediate neighbours | ykzO, tnrA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13300
Phenotypes of a mutant
- a mgtE mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
- a mgtE yfjQ mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
Database entries
- BsubCyc: BSU13300
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: SLC41A transporter family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU13300
- Structure: 2QBZ (the mgtE riboswitch)
- UniProt: O34442
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism: M-box: Mg(2+)-responsive riboswitch PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Catherine A Wakeman, Jonathan R Goodson, Vineetha M Zacharia, Wade C Winkler
Assessment of the requirements for magnesium transporters in Bacillus subtilis.
J Bacteriol: 2014, 196(6);1206-14
[PubMed:24415722]
[WorldCat.org]
[DOI]
(I p)
Jaya Sahni, Yumei Song, Andrew M Scharenberg
The B. subtilis MgtE magnesium transporter can functionally compensate TRPM7-deficiency in vertebrate B-cells.
PLoS One: 2012, 7(9);e44452
[PubMed:22970223]
[WorldCat.org]
[DOI]
(I p)
Arati Ramesh, Catherine A Wakeman, Wade C Winkler
Insights into metalloregulation by M-box riboswitch RNAs via structural analysis of manganese-bound complexes.
J Mol Biol: 2011, 407(4);556-70
[PubMed:21315082]
[WorldCat.org]
[DOI]
(I p)
Charles E Dann, Catherine A Wakeman, Cecelia L Sieling, Stephanie C Baker, Irnov Irnov, Wade C Winkler
Structure and mechanism of a metal-sensing regulatory RNA.
Cell: 2007, 130(5);878-92
[PubMed:17803910]
[WorldCat.org]
[DOI]
(P p)
Jeffrey E Barrick, Keith A Corbino, Wade C Winkler, Ali Nahvi, Maumita Mandal, Jennifer Collins, Mark Lee, Adam Roth, Narasimhan Sudarsan, Inbal Jona, J Kenneth Wickiser, Ronald R Breaker
New RNA motifs suggest an expanded scope for riboswitches in bacterial genetic control.
Proc Natl Acad Sci U S A: 2004, 101(17);6421-6
[PubMed:15096624]
[WorldCat.org]
[DOI]
(P p)
