Difference between revisions of "TrpS"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11420&redirect=T BSU11420]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpS.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpS.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU11420&redirect=T BSU11420]
  
 
* '''Structure:'''  
 
* '''Structure:'''  

Revision as of 13:24, 2 April 2014

  • Description: tryptophanyl-tRNA synthetase

Gene name trpS
Synonyms
Essential yes PubMed
Product tryptophanyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: trpS
Metabolic function and regulation of this protein in SubtiPathways:
trpS
MW, pI 37 kDa, 6.542
Gene length, protein length 990 bp, 330 aa
Immediate neighbours yjbA, oppA
Sequences Protein DNA DNA_with_flanks
Genetic context
TrpS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TrpS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU11420

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 3PRH (Val144Pro mutant) PubMed
    • 1BM2 (complex with tryptophan, Geobacillus stearothermophilus)
    • 1MAW (complex with ATP, Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
    • T-box: transcriptional antitermination in the absence of tryptophan (upon tRNA-binding to the mRNA leader region) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References