NasF

• Description: uroporphyrinogen methyltransferase
  
  

Gene name	nasF
Synonyms	nasBE
Essential	no
Product	uroporphyrinogen methyltransferase
Function	nitrate respiration
Gene expression levels in SubtiExpress: nasF
Metabolic function and regulation of this protein in SubtiPathways: nasF
MW, pI	53 kDa, 6.523
Gene length, protein length	1449 bp, 483 aa
Immediate neighbours	ycgT, nasE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

Fur regulon, NsrR regulon, ResD regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU03280

Database entries

• BsubCyc: BSU03280

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1 (according to Swiss-Prot)

• Protein family: precorrin methyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU03280

• Structure:

• UniProt: P42437

• KEGG entry: [3]

• E.C. number: 2.1.1.107

Expression and regulation

• Operon:
  • nasB-nasC-nasD-nasE-nasF PubMed
  • nasD-nasE-nasF PubMed

• Expression browser: nasF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • nasD: expressed under anaerobic conditions (ResD) PubMed
  • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
  • induced by iron starvation (Fur) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • ResD: transcription activation PubMed
  • NsrR: transcription repression PubMed
  • Fur: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536] [WorldCat.org] [DOI] (I p)