Difference between revisions of "RadA"
| Line 1: | Line 1: | ||
| − | * '''Description:''' negative effector of [[DisA]] activity, suppressor of ''[[recU]]'' and ''[[recB]]'' mutations, participates in the stabilization and or processing of holliday junction intermediates <br/><br/> | + | * '''Description:''' 6-O-methylguanine-DNA methyltransferase, negative effector of [[DisA]] activity, suppressor of ''[[recU]]'' and ''[[recB]]'' mutations, participates in the stabilization and or processing of holliday junction intermediates <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA | + | |style="background:#ABCDEF;" align="center"| '''Product''' || 6-O-methylguanine-DNA methyltransferase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || homologous recombination , control of c-di-AMP formation | |style="background:#ABCDEF;" align="center"|'''Function''' || homologous recombination , control of c-di-AMP formation | ||
Revision as of 12:49, 2 April 2014
- Description: 6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, suppressor of recU and recB mutations, participates in the stabilization and or processing of holliday junction intermediates
| Gene name | radA |
| Synonyms | sms, yacJ |
| Essential | no |
| Product | 6-O-methylguanine-DNA methyltransferase |
| Function | homologous recombination , control of c-di-AMP formation |
| Gene expression levels in SubtiExpress: radA | |
| Interactions involving this protein in SubtInteract: RadA | |
| Metabolic function and regulation of this protein in SubtiPathways: radA | |
| MW, pI | 49 kDa, 8.128 |
| Gene length, protein length | 1374 bp, 458 aa |
| Immediate neighbours | clpC, disA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides, DNA repair/ recombination, general stress proteins (controlled by SigB), heat shock proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00870
Phenotypes of a mutant
Database entries
- BsubCyc: BSU00870
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: RadA subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU00870
- Structure:
- UniProt: P37572
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- BKG1 (radA::spc), available in Jörg Stülke's lab
- BKG2 (radA-disA::spc), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lei Zhang, Zheng-Guo He
Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic Di-AMP synthesis activity in Mycobacterium smegmatis.
J Biol Chem: 2013, 288(31);22426-36
[PubMed:23760274]
[WorldCat.org]
[DOI]
(I p)
Begoña Carrasco, M Castillo Cozar, Rudi Lurz, Juan C Alonso, Silvia Ayora
Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation.
J Bacteriol: 2004, 186(17);5557-66
[PubMed:15317759]
[WorldCat.org]
[DOI]
(P p)
A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224]
[WorldCat.org]
[DOI]
(P p)
I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115]
[WorldCat.org]
[DOI]
(P p)
E Krüger, T Msadek, M Hecker
Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.
Mol Microbiol: 1996, 20(4);713-23
[PubMed:8793870]
[WorldCat.org]
[DOI]
(P p)
