YwaD

• Description: double-zinc aminopeptidase
  
  

Gene name	ywaD
Synonyms	ipa-8r
Essential	no
Product	double-zinc aminopeptidase
Function	unknown
Gene expression levels in SubtiExpress: ywaD
MW, pI	49 kDa, 6.638
Gene length, protein length	1365 bp, 455 aa
Immediate neighbours	tyrZ, sasA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU38470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: M28B subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure:

• UniProt: P25152

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ywaD PubMed

• Expression browser: ywaD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xinxing Gao, Zhongmei Liu, Wenjing Cui, Li Zhou, Yaping Tian, Zhemin Zhou
Enhanced thermal stability and hydrolytic ability of Bacillus subtilis aminopeptidase by removing the thermal sensitive domain in the non-catalytic region.
PLoS One: 2014, 9(3);e92357
[PubMed:24633010] [WorldCat.org] [DOI] (I e)

Xinxing Gao, Wenjing Cui, Ning Ding, Zhongmei Liu, Yaping Tian, Zhemin Zhou
Structure-based approach to alter the substrate specificity of Bacillus subtilis aminopeptidase.
Prion: 2013, 7(4);328-34
[PubMed:23787698] [WorldCat.org] [DOI] (I p)

Xinxing Gao, Wenjing Cui, Yaping Tian, Zhemin Zhou
Over-expression, secretion, biochemical characterisation, and structure analysis of Bacillus subtilis aminopeptidase.
J Sci Food Agric: 2013, 93(11);2810-5
[PubMed:23426795] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Yifat Fundoiano-Hershcovitz, Larisa Rabinovitch, Smadar Shulami, Vera Reiland, Gil Shoham, Yuval Shoham
The ywad gene from Bacillus subtilis encodes a double-zinc aminopeptidase.
FEMS Microbiol Lett: 2005, 243(1);157-63
[PubMed:15668014] [WorldCat.org] [DOI] (P p)