Difference between revisions of "YhcA"

From SubtiWiki
Jump to: navigation, search
Line 59: Line 59:
  
 
=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
Line 77: Line 74:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
Line 119: Line 116:
  
 
* '''Mutant:'''
 
* '''Mutant:'''
 +
** GP1704 (''[[yhcA]]''::''ermC'', without terminator), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:01, 14 March 2014

  • Description: similar to multidrug resistance protein

Gene name yhcA
Synonyms
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yhcA
MW, pI 58 kDa, 9.49
Gene length, protein length 1596 bp, 532 aa
Immediate neighbours yhbJ, yhcB
Sequences Protein DNA DNA_with_flanks
Genetic context
YhcA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YhcA expression.png















Categories containing this gene/protein

transporters/ other, resistance against toxins/ antibiotics, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU09010

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: EmrB family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 4 to 41 min) PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947] [WorldCat.org] [DOI] (I p)