Difference between revisions of "GroEL"
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{{SubtiWiki category|[[heat shock proteins]]}}, | {{SubtiWiki category|[[heat shock proteins]]}}, | ||
{{SubtiWiki category|[[essential genes]]}}, | {{SubtiWiki category|[[essential genes]]}}, | ||
| − | {{SubtiWiki category|[[phosphoproteins]]}} | + | {{SubtiWiki category|[[phosphoproteins]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
| − | |||
| − | |||
| − | |||
=The protein= | =The protein= | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
| − | <pubmed>9303302,1350777,12082092,,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed> | + | <pubmed>9303302,1350777,12082092,15378759,17726680 16493705 9303302,1350776 ,8576042 7592421 22517742 22268681 24263382</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 13:00, 5 March 2014
- Description: chaperonin and co-repressor for HrcA
| Gene name | groEL |
| Synonyms | |
| Essential | yes PubMed |
| Product | chaperonin, co-repressor for HrcA |
| Function | protein folding and re-folding |
| Gene expression levels in SubtiExpress: groEL | |
| Interactions involving this protein in SubtInteract: GroEL | |
| Metabolic function and regulation of this protein in SubtiPathways: groEL | |
| MW, pI | 57 kDa, 4.531 |
| Gene length, protein length | 1632 bp, 544 aa |
| Immediate neighbours | groES, ydzT/1 |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
chaperones/ protein folding, heat shock proteins, essential genes, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU06030
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: acts as co-repressor for HrcA
- Protein family: chaperonin (HSP60) family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
- recruited to the membrane after ethanol stress PubMed
Database entries
- Structure:
- UniProt: P28598
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- Expression vector:
- for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1000 available in Jörg Stülke's lab
- for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP1002 available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Wolfgang Schumann, Bayreuth University, Germany Homepage
Your additional remarks
References
