Difference between revisions of "Pgi"

From SubtiWiki
Jump to: navigation, search
Line 63: Line 63:
  
 
=== Additional information===
 
=== Additional information===
 
 
  
 
=The protein=
 
=The protein=
Line 78: Line 76:
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
  
* '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/7400125 PubMed]
+
* '''Kinetic information:'''  
 +
** Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/7400125 PubMed]
 +
** specific activity: 2.1 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}}
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:''' phosphorylation on Thr-39 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
 
* '''Modification:''' phosphorylation on Thr-39 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:''' competitively inhibited by 6-phosphogluconate (in ''B.caldotenax, B.stearothermophilus'') [http://www.ncbi.nlm.nih.gov/pubmed/7400125 PubMed]
 
* '''Effectors of protein activity:''' competitively inhibited by 6-phosphogluconate (in ''B.caldotenax, B.stearothermophilus'') [http://www.ncbi.nlm.nih.gov/pubmed/7400125 PubMed]
Line 139: Line 139:
  
 
=References=
 
=References=
<pubmed>17218307 19052382 4214896 23420519 11489127 4275311 11491085 17218307, </pubmed>
+
<pubmed>17218307 19052382 4214896 23420519 11489127 4275311 11491085 17218307, 24571712 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:27, 28 February 2014

  • Description: glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme

Gene name pgi
Synonyms yugL
Essential no
Product glucose-6-phosphate isomerase
Function enzyme in glycolysis / gluconeogenesis
Gene expression levels in SubtiExpress: pgi
Metabolic function and regulation of this protein in SubtiPathways:
pgi
MW, pI 50.4 kDa, 4.85
Gene length, protein length 1353 bp, 451 amino acids
Immediate neighbours yugM, yugK
Sequences Protein DNA DNA_with_flanks
Genetic context
Pgi context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Pgi expression.png















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU31350

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate
  • Protein family: GPI family (according to Swiss-Prot) GPI family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • Reversible Michaelis-Menten PubMed
    • specific activity: 2.1 µmol min-1 (mg protein)-1 PubMed
  • Modification: phosphorylation on Thr-39 PubMed
  • Effectors of protein activity: competitively inhibited by 6-phosphogluconate (in B.caldotenax, B.stearothermophilus) PubMed
  • Localization:
    • cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

  • Structure: 1B0Z (Geobacillus stearothermophilus), 2PGI (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of Pgi can be found at Proteopedia

Expression and regulation

  • Regulation: constitutively expressed PubMed
  • Additional information:

Biological materials

  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany homepage

Your additional remarks

References