Difference between revisions of "MgtE"
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| − | * '''Description:''' magnesium | + | * '''Description:''' primary magnesium transporter <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || magnesium | + | |style="background:#ABCDEF;" align="center"| '''Product''' || primary magnesium transporter |
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|style="background:#ABCDEF;" align="center"|'''Function''' || magnesium uptake | |style="background:#ABCDEF;" align="center"|'''Function''' || magnesium uptake | ||
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * a ''[[mgtE]]'' mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg<sup>2+</sup>, moreover the mutant grows well on minimal medim in the presence of citrate {{PubMed|24415722}} | ||
| + | * a ''[[mgtE]] [[yfjQ]]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg<sup>2+</sup>, moreover the mutant grows well on minimal medim in the presence of citrate {{PubMed|24415722}} | ||
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgtE_1396013_1397368_1 mgtE] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mgtE_1396013_1397368_1 mgtE] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' induced at low magnesium concentration ([[M-box]]) {{PubMed|17803910}} | * '''Regulation:''' induced at low magnesium concentration ([[M-box]]) {{PubMed|17803910}} | ||
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=References= | =References= | ||
| − | <pubmed>17803910,15096624, 21315082, 22970223 </pubmed> | + | <pubmed>17803910,15096624, 21315082, 22970223 24415722</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:10, 16 January 2014
- Description: primary magnesium transporter
| Gene name | mgtE |
| Synonyms | ykoK |
| Essential | no |
| Product | primary magnesium transporter |
| Function | magnesium uptake |
| Gene expression levels in SubtiExpress: mgtE | |
| MW, pI | 50 kDa, 4.609 |
| Gene length, protein length | 1353 bp, 451 aa |
| Immediate neighbours | ykzO, tnrA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13300
Phenotypes of a mutant
- a mgtE mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
- a mgtE yfjQ mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medim in the presence of citrate PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: SLC41A transporter family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 2QBZ (the mgtE riboswitch)
- UniProt: O34442
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism: M-box: Mg(2+)-responsive riboswitch PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
