Difference between revisions of "BglS"

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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP427 (licT-bglS, erm), BGW7 (cat), both available in the [[Stülke]] lab
+
* '''Mutant:''' GP427 (''[[licT]]-[[bglS]]'', erm), BGW7 (cat), both available in the [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
  
<pubmed>18957862 12850135 6087283 8245830 8245831 8606172, 23459129 </pubmed>
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<pubmed>18957862 12850135 6087283 8245830 8245831 8606172, 23459129 24391357 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:22, 7 January 2014

  • Description: endo-beta-1,3-1,4 glucanase

Gene name bglS
Synonyms bgl, licS
Essential no
Product endo-beta-1,3-1,4 glucanase
Function lichenan degradation
Gene expression levels in SubtiExpress: bglS
Metabolic function and regulation of this protein in SubtiPathways:
bglS
MW, pI 27 kDa, 6.482
Gene length, protein length 726 bp, 242 aa
Immediate neighbours citH, licT
Sequences Protein DNA DNA_with_flanks
Genetic context
BglS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BglS expression.png















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, LicT regulon

The gene

Basic information

  • Locus tag: BSU39070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 16 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (3.1-fold) (CcpA) PubMed
    • expressed in the stationary phase (temporal activation) PubMed
    • weak induction by ß-glucosides (LicT) PubMed
  • Additional information:

Biological materials

  • Mutant: GP427 (licT-bglS, erm), BGW7 (cat), both available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References