BglS

• Description: endo-beta-1,3-1,4 glucanase
  
  

Gene name	bglS
Synonyms	bgl, licS
Essential	no
Product	endo-beta-1,3-1,4 glucanase
Function	lichenan degradation
Gene expression levels in SubtiExpress: bglS
Metabolic function and regulation of this protein in SubtiPathways: bglS
MW, pI	27 kDa, 6.482
Gene length, protein length	726 bp, 242 aa
Immediate neighbours	citH, licT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, LicT regulon

The gene

Basic information

• Locus tag: BSU39070

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 16 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure: 3O5S

• UniProt: P04957

• KEGG entry: [3]

• E.C. number: 3.2.1.73

Expression and regulation

• Operon:
  • licT-bglS PubMed
  • bglS PubMed

• Expression browser: bglS PubMed

• Sigma factor:
  • licT: SigA PubMed

• Regulation:
  • repressed by glucose (3.1-fold) (CcpA) PubMed
  • expressed in the stationary phase (temporal activation) PubMed
  • weak induction by ß-glucosides (LicT) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LicT: binding to an RNA switch results in transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant: GP427 (licT-bglS, erm), BGW7 (cat), both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: