Difference between revisions of "MrpD"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
+
* essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
+
* a ''[[mrpD]]'' mutant is viable at pH7.4 and sodium concentrations below 80 mM {{PubMed|24139955}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 15:28, 22 December 2013

  • Description: H+ transporter subunit of the Na+/H+ antiporter, multiple resistance and pH homeostasis

Gene name mrpD
Synonyms yufD
Essential yes PubMed
Product Na+/H+ antiporter subunit
Function sodium export
Gene expression levels in SubtiExpress: mrpD
Interactions involving this protein in SubtInteract: MrpD
MW, pI 53 kDa, 9.488
Gene length, protein length 1479 bp, 493 aa
Immediate neighbours mrpC, mrpE
Sequences Protein DNA DNA_with_flanks
Genetic context
MrpD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MrpD expression.png















Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), essential genes, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU31630

Phenotypes of a mutant

  • essential PubMed
  • a mrpD mutant is viable at pH7.4 and sodium concentrations below 80 mM PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: H+ transporter subunit of the Na+/H+ antiporter PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Vamsi K Moparthi, Brijesh Kumar, Cecilie Mathiesen, Cecilia Hägerhäll
Homologous protein subunits from Escherichia coli NADH:quinone oxidoreductase can functionally replace MrpA and MrpD in Bacillus subtilis.
Biochim Biophys Acta: 2011, 1807(4);427-36
[PubMed:21236240] [WorldCat.org] [DOI] (P p)

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Toshiaki Kudo, Saori Kosono
The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
Microbiology (Reading): 2009, 155(Pt 7);2137-2147
[PubMed:19389778] [WorldCat.org] [DOI] (P p)

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Saori Kosono, Toshiaki Kudo
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
J Bacteriol: 2007, 189(20);7511-4
[PubMed:17693497] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, W Wang, T A Krulwich
Effects of nonpolar mutations in each of the seven Bacillus subtilis mrp genes suggest complex interactions among the gene products in support of Na(+) and alkali but not cholate resistance.
J Bacteriol: 2000, 182(20);5663-70
[PubMed:11004162] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, B Oudega, T A Krulwich
mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis.
J Bacteriol: 1999, 181(8);2394-402
[PubMed:10198001] [WorldCat.org] [DOI] (P p)