UgtP

• Description: UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of cell division
  
  

Gene name	ugtP
Synonyms	ypfP
Essential	no
Product	UDP-glucose diacylglycerol glucosyltransferase
Function	synthesis of glycolipids and anchoring of lipoteichoic acid, inhibition of FtsZ assembly
Gene expression levels in SubtiExpress: ugtP
Interactions involving this protein in SubtInteract: UgtP
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	43 kDa, 8.398
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	metA, cspD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, lipid metabolism/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU21920

Phenotypes of a mutant

• cells are bent and distended PubMed
• increased expression of the SigM, SigV, and SigX regulons PubMed
• altered localization of MreB (irregular clusters instead of helical dots) PubMed
• the inactivation of ugtP suppresses the poor and filametous growth of the yvcL zapA double mutant PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot)
  • the interaction with FtsZ results in inhibition of cell division and an increase of cell size PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • oligomerization of UgtP is inhibited by UDP-Glc and by interaction with FtsZ PubMed

• Interactions:
  • EzrA-UgtP PubMed
  • UgtP-UgtP PubMed, forms oligomers PubMed
  • UgtP-FtsZ (under nutrient rich conditions) PubMed

• Localization:
  • membrane-bound protein, self-assembles into tightly wound spirals in vitro PubMed
  • under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with FtsZ PubMed
  • under nutrient poor conditions: forms punctate foci (oligomers), no interaction with FtsZ PubMed

Database entries

• Structure:

• UniProt: P54166

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • metA-ugtP PubMed
  • ugtP PubMed

• Expression browser: ugtP PubMed

• Sigma factor:
  • ugtP: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP1369 (ugtP::spc), available in Jörg Stülke's lab

• Expression vector:
  • pGP2571, for expression in B. subtilis (based on pBQ200, available in Jörg Stülke's lab
  • pGP2600, for expression/ purification from E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: