Difference between revisions of "YvcL"
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| − | * '''Description:''' WhiA family protein | + | * '''Description:''' WhiA family protein, involved in Z ring assembly, putative transcription factor<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[cell division]] protein |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || control of Z ring asembly |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34750 yvcL] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34750 yvcL] | ||
| Line 38: | Line 38: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[cell division]]}}, | ||
{{SubtiWiki category|[[transcription factors and their control]]}} | {{SubtiWiki category|[[transcription factors and their control]]}} | ||
| Line 50: | Line 51: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| + | * a ''[[yvcL]] [[zapA]]'' double mutant grows poorly, and the cells are filamentous {{PubMed|24097947}} | ||
=== Database entries === | === Database entries === | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' suggested HTH domain [http://www.ncbi.nlm.nih.gov/sites/entrez/19836336 PubMed] | + | * '''[[Domains]]:''' suggested HTH domain [http://www.ncbi.nlm.nih.gov/sites/entrez/19836336 PubMed] |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 90: | Line 92: | ||
=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3HYI 3HYI] (Crystal structure of full-length DUF199/WhiA from ''Thermotoga maritima'') | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=3HYI 3HYI] (Crystal structure of full-length DUF199/WhiA from ''Thermotoga maritima'') | ||
| + | ** [http://www.rcsb.org/pdb/explore.do?structureId=3HYJ 3HYJ] (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA) | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/O06975 O06975] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O06975 O06975] | ||
Revision as of 17:52, 21 December 2013
- Description: WhiA family protein, involved in Z ring assembly, putative transcription factor
| Gene name | yvcL |
| Synonyms | |
| Essential | no |
| Product | cell division protein |
| Function | control of Z ring asembly |
| Gene expression levels in SubtiExpress: yvcL | |
| MW, pI | 36 kDa, 9.479 |
| Gene length, protein length | 948 bp, 316 aa |
| Immediate neighbours | crh, yvcK |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell division, transcription factors and their control
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34750
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: WhiA family PubMed
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O06975
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP231 (amyE::PrpsJ-lacZ, yvcL::cat) available in Jörg Stülke's lab; plasmid pGP1468 was used to disrupt the gene.
- Expression vector: pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
WhiA orthologues are present in all Gram-positive bacteria and best characterized by the WhiA sporulation control factor in Streptomyces coelicolor. They are thought to act as genetic regulators that have evolved from LAGLIDADG homing endonucleases (LHE).
References
