Difference between revisions of "UgtP"
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<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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** under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with [[FtsZ]] {{PubMed|22931116}} | ** under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with [[FtsZ]] {{PubMed|22931116}} | ||
** under nutrient poor conditions: forms punctate foci (oligomers), no interaction with [[FtsZ]] {{PubMed|22931116}} | ** under nutrient poor conditions: forms punctate foci (oligomers), no interaction with [[FtsZ]] {{PubMed|22931116}} | ||
| + | |||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ugtP_2306514_2307662_1 ugtP] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ugtP_2306514_2307662_1 ugtP] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed] | + | * '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 PubMed] |
* '''Regulation:''' | * '''Regulation:''' | ||
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* '''Expression vector:''' | * '''Expression vector:''' | ||
| − | **pGP2571 (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab | + | ** pGP2571, for expression in ''B. subtilis'' (based on [[pBQ200]], available in [[Jörg Stülke]]'s lab |
| + | ** pGP2600, for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Jörg Stülke]]'s lab | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
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==Reviews== | ==Reviews== | ||
<pubmed> 19680248 17662935 22575476 </pubmed> | <pubmed> 19680248 17662935 22575476 </pubmed> | ||
| − | |||
==Original Publications== | ==Original Publications== | ||
<pubmed>9244290,18820022,17662947,9720862 22362028 15640167 23935518 22931116</pubmed> | <pubmed>9244290,18820022,17662947,9720862 22362028 15640167 23935518 22931116</pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 17:24, 14 December 2013
- Description: UDP-glucose diacylglycerol glucosyltransferase, growth-rate dependent inhibitor of cell division
| Gene name | ugtP |
| Synonyms | ypfP |
| Essential | no |
| Product | UDP-glucose diacylglycerol glucosyltransferase |
| Function | synthesis of glycolipids and anchoring of lipoteichoic acid, inhibition of FtsZ assembly |
| Gene expression levels in SubtiExpress: ugtP | |
| Interactions involving this protein in SubtInteract: UgtP | |
| Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis | |
| MW, pI | 43 kDa, 8.398 |
| Gene length, protein length | 1146 bp, 382 aa |
| Immediate neighbours | metA, cspD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell division, lipid metabolism/ other, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU21920
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- UDP-glucose + 1,2-diacylglycerol = UDP + 1,2-diacyl-3-(O-beta-D-glucopyranosyl)-sn-glycerol (according to Swiss-Prot)
- the interaction with FtsZ results in inhibition of cell division and an increase of cell size PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization:
- membrane-bound protein, self-assembles into tightly wound spirals in vitro PubMed
- under nutrient rich conditions (increased concentration of UDP-Glc): throughout the cell, concentrated at the cell poles and/or the cytokinetic ring, interaction with FtsZ PubMed
- under nutrient poor conditions: forms punctate foci (oligomers), no interaction with FtsZ PubMed
Database entries
- Structure:
- UniProt: P54166
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1369 (ugtP::spc), available in Jörg Stülke's lab
- Expression vector:
- pGP2571, for expression in B. subtilis (based on pBQ200, available in Jörg Stülke's lab
- pGP2600, for expression/ purification from E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Publications
