Difference between revisions of "DisA"
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| − | * '''Description:''' DNA integrity scanning protein, delays sporulation in the case of chromosome damage, | + | * '''Description:''' DNA integrity scanning protein,diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA [[replication]] during ''B. subtilis'' spore outgrowth until the germinating spore's genome is free of damage <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Product''' || DNA integrity scanning protein, has diadenylate cyclase activity | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA integrity scanning protein, has diadenylate cyclase activity | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || control of sporulation initiation | + | |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[sporulation]] initiation |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00880 disA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00880 disA] | ||
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* '''[[SubtInteract|Interactions]]:''' | * '''[[SubtInteract|Interactions]]:''' | ||
| + | ** forms octamers | ||
** [[RacA]]-[[DisA]] {{PubMed|12493822}} | ** [[RacA]]-[[DisA]] {{PubMed|12493822}} | ||
** [[RadA]]-[[DisA]] {{PubMed|23760274}}, this interaction result in the inhibition of [[DisA]] activity {{PubMed|23760274}} | ** [[RadA]]-[[DisA]] {{PubMed|23760274}}, this interaction result in the inhibition of [[DisA]] activity {{PubMed|23760274}} | ||
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* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
** see a [http://download.cell.com/mmcs/journals/0092-8674/PIIS0092867406005034.mmc12.avi video] showing the movement of DisA in the cell (in real time) [http://www.ncbi.nlm.nih.gov/pubmed/16713562 PubMed] | ** see a [http://download.cell.com/mmcs/journals/0092-8674/PIIS0092867406005034.mmc12.avi video] showing the movement of DisA in the cell (in real time) [http://www.ncbi.nlm.nih.gov/pubmed/16713562 PubMed] | ||
| + | ** forms discrete globular foci in germinating spres that colocalize with the nucleoid {{PubMed|24244006}} | ||
=== Database entries === | === Database entries === | ||
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** ''disA'': [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed] | ** ''disA'': [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17434969 PubMed] | ||
| − | * '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed], induced by heat ([[CtsR]]) [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed] | + | * '''Regulation:''' |
| + | ** induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed], induced by heat ([[CtsR]]) [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed] | ||
| + | ** expressed during [[sporulation]] in the mother cell {{PubMed|24244006}} | ||
| + | ** expressed during germination and spore outgrowth {{PubMed|24244006}} | ||
* '''Regulatory mechanism:''' [[CtsR]]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed] | * '''Regulatory mechanism:''' [[CtsR]]: transcription repression [http://www.ncbi.nlm.nih.gov/pubmed/9987115 PubMed] | ||
Revision as of 12:57, 27 November 2013
- Description: DNA integrity scanning protein,diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA replication during B. subtilis spore outgrowth until the germinating spore's genome is free of damage
| Gene name | disA |
| Synonyms | yacK |
| Essential | no |
| Product | DNA integrity scanning protein, has diadenylate cyclase activity |
| Function | control of sporulation initiation |
| Gene expression levels in SubtiExpress: disA | |
| Interactions involving this protein in SubtInteract: DisA | |
| Metabolic function and regulation of this protein in SubtiPathways: Stress | |
| MW, pI | 40 kDa, 5.569 |
| Gene length, protein length | 1080 bp, 360 aa |
| Immediate neighbours | radA, yacL |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell division, metabolism of signalling nucleotides, sporulation/ other, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), heat shock proteins
This gene is a member of the following regulons
CtsR regulon, SigB regulon, SigM regulon
The gene
Basic information
- Locus tag: BSU00880
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- synthesis of c-di-AMP from two molecules of ATP PubMed
- Protein family: disA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
Database entries
- Structure: 3C23
- UniProt: P37573
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Expression vector:
- IPTG inducible expression of His-disA in E. coli: pGP2563 (in pET19b), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
