Difference between revisions of "ThiI"
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of 4-thiouridine in tRNA | |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of 4-thiouridine in tRNA | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=ThiI ThiI] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 9.663 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 9.663 | ||
Revision as of 14:19, 18 November 2013
- Description: sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA
| Gene name | thiI |
| Synonyms | ytbJ |
| Essential | no |
| Product | sulfuryl transferase |
| Function | biosynthesis of 4-thiouridine in tRNA |
| Interactions involving this protein in SubtInteract: ThiI | |
| MW, pI | 40 kDa, 9.663 |
| Gene length, protein length | 1074 bp, 358 aa |
| Immediate neighbours | sspA, nifZ |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29580
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: receives a sulfuryl group from NifZ and transfers it to uridine at position 8 of tRNA PubMed
- Protein family: thiI family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O34595
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787]
[WorldCat.org]
[DOI]
(I p)
Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704]
[WorldCat.org]
[DOI]
(P p)
