RpoE

• Description: RNA polymerase delta subunit, affects the regulation of RNA polymerase by the concentration of the initiating nucleoside triphosphate ([iNTP])
  
  

Gene name	rpoE
Synonyms
Essential	no
Product	RNA polymerase delta subunit
Function	transcription
Gene expression levels in SubtiExpress: rpoE
Interactions involving this protein in SubtInteract: RpoE
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	20 kDa, 3.654
Gene length, protein length	519 bp, 173 aa
Immediate neighbours	pyrG, acdA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription

This gene is a member of the following regulons

FadR regulon

The gene

Basic information

• Locus tag: BSU37160

Phenotypes of a mutant

• RpoE is essential for cell survival when facing a competing strain in changing environment PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: rpoE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • closely associated to RNA polymerase (RpoA(2)-RpoB-RpoC) PubMed

• Localization:
  • closely associated with RNA polymerase involved in transcribing both mRNA and rRNA operons PubMed

Database entries

• Structure:
  • 2KRC (N-terminal domain) PubMed
  • 2M4K (full-length protein) PubMed

• UniProt: P12464

• KEGG entry: [3]

• E.C. number: 2.7.7.6

Expression and regulation

• Operon:
  • fadF-acdA-rpoE PubMed
  • rpoE PubMed

• Expression browser: rpoE PubMed

• Sigma factor:
  • fadF: SigA PubMed
  • rpoE: SigA PubMed

• Regulation:
  • fadF: repressed in the absence of long-chain fatty acids (FadR) PubMed

• Regulatory mechanism:
  • FadR: transcription repression PubMed

• Additional information:
  • present at equimolar levels with RNA polymerase PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage

References

Reviews

Lakshminarayan M Iyer, L Aravind
Insights from the architecture of the bacterial transcription apparatus.
J Struct Biol: 2012, 179(3);299-319
[PubMed:22210308] [WorldCat.org] [DOI] (I p)

Original publications

Veronika Papoušková, Pavel Kadeřávek, Olga Otrusinová, Alžbeta Rabatinová, Hana ŠSanderová, Jiří Nováček, Libor Krásný, Vladimír Sklenář, Lukáš Žídek
Structural study of the partially disordered full-length δ subunit of RNA polymerase from Bacillus subtilis.
Chembiochem: 2013, 14(14);1772-9
[PubMed:23868186] [WorldCat.org] [DOI] (I p)

Alžbeta Rabatinová, Hana Šanderová, Jitka Jirát Matějčková, Jana Korelusová, Luděk Sojka, Ivan Barvík, Veronika Papoušková, Vladimír Sklenár, Lukáš Žídek, Libor Krásný
The δ subunit of RNA polymerase is required for rapid changes in gene expression and competitive fitness of the cell.
J Bacteriol: 2013, 195(11);2603-11
[PubMed:23543716] [WorldCat.org] [DOI] (I p)

Anna Zawadzka-Kazimierczuk, Wiktor Koźmiński, Hana Sanderová, Libor Krásný
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins.
J Biomol NMR: 2012, 52(4);329-37
[PubMed:22350953] [WorldCat.org] [DOI] (I p)

Jiří Nováček, Anna Zawadzka-Kazimierczuk, Veronika Papoušková, Lukáš Zídek, Hana Sanderová, Libor Krásný, Wiktor Koźmiński, Vladimír Sklenář
5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion.
J Biomol NMR: 2011, 50(1);1-11
[PubMed:21424579] [WorldCat.org] [DOI] (I p)

Veronika Motáčková, Jiří Nováček, Anna Zawadzka-Kazimierczuk, Krzysztof Kazimierczuk, Lukáš Zídek, Hana Sanderová, Libor Krásný, Wiktor Koźmiński, Vladimír Sklenář
Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments.
J Biomol NMR: 2010, 48(3);169-77
[PubMed:20890634] [WorldCat.org] [DOI] (I p)

Geoff P Doherty, Mark J Fogg, Anthony J Wilkinson, Peter J Lewis
Small subunits of RNA polymerase: localization, levels and implications for core enzyme composition.
Microbiology (Reading): 2010, 156(Pt 12);3532-3543
[PubMed:20724389] [WorldCat.org] [DOI] (I p)

Veronika Motácková, Hana Sanderová, Lukás Zídek, Jirí Novácek, Petr Padrta, Alzbeta Svenková, Jana Korelusová, Jirí Jonák, Libor Krásný, Vladimír Sklenár
Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs.
Proteins: 2010, 78(7);1807-10
[PubMed:20310067] [WorldCat.org] [DOI] (I p)

Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250] [WorldCat.org] [DOI] (P p)

F J López de Saro, N Yoshikawa, J D Helmann
Expression, abundance, and RNA polymerase binding properties of the delta factor of Bacillus subtilis.
J Biol Chem: 1999, 274(22);15953-8
[PubMed:10336502] [WorldCat.org] [DOI] (P p)

F J López de Saro, A Y Woody, J D Helmann
Structural analysis of the Bacillus subtilis delta factor: a protein polyanion which displaces RNA from RNA polymerase.
J Mol Biol: 1995, 252(2);189-202
[PubMed:7545758] [WorldCat.org] [DOI] (P p)

Y L Juang, J D Helmann
Pathway of promoter melting by Bacillus subtilis RNA polymerase at a stable RNA promoter: effects of temperature, delta protein, and sigma factor mutations.
Biochemistry: 1995, 34(26);8465-73
[PubMed:7599136] [WorldCat.org] [DOI] (P p)

Y L Juang, J D Helmann
The delta subunit of Bacillus subtilis RNA polymerase. An allosteric effector of the initiation and core-recycling phases of transcription.
J Mol Biol: 1994, 239(1);1-14
[PubMed:7515111] [WorldCat.org] [DOI] (P p)

M Lampe, C Binnie, R Schmidt, R Losick
Cloned gene encoding the delta subunit of Bacillus subtilis RNA polymerase.
Gene: 1988, 67(1);13-9
[PubMed:2843435] [WorldCat.org] [DOI] (P p)

E I Hyde, M D Hilton, H R Whiteley
Interactions of Bacillus subtilis RNA polymerase with subunits determining the specificity of initiation. Sigma and delta peptides can bind simultaneously to core.
J Biol Chem: 1986, 261(35);16565-70
[PubMed:3097010] [WorldCat.org] (P p)

E C Achberger, H R Whiteley
The role of the delta peptide of the Bacillus subtilis RNA polymerase in promoter selection.
J Biol Chem: 1981, 256(14);7424-32
[PubMed:6788769] [WorldCat.org] (P p)