Difference between revisions of "YloU"

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(Biological materials)
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
essential [http://www.ncbi.nlm.nih.gov/pubmed/17114254 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Mutant:
 
* '''Mutant:
** GP1465 (''yloU''::''cat''), available in [[Jörg Stülke]]'s lab
+
** GP1465 (''yloU''::''cat''), available in [[Jörg Stülke]]'s lab {{PubMed|24178028}}
 
** GP1467 (''yloU - [[yloV]]''::''cat''), available in [[Jörg Stülke]]'s lab
 
** GP1467 (''yloU - [[yloV]]''::''cat''), available in [[Jörg Stülke]]'s lab
  
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=References=
 
=References=
  
<pubmed>17114254, 22383849 23420519 </pubmed>
+
<pubmed>17114254, 22383849 23420519 24178028 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:58, 2 November 2013

  • Description: similar to alkaline-shock protein

Gene name yloU
Synonyms
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yloU
MW, pI 13 kDa, 7.077
Gene length, protein length 360 bp, 120 aa
Immediate neighbours rpmB, yloV
Sequences Protein DNA DNA_with_flanks
Genetic context
YloU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YloU expression.png















Categories containing this gene/protein

proteins of unknown function, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15830

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: asp23 family (according to Swiss-Prot)
  • Paralogous protein(s): YqhY

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • GP1476 (chromosomal yloU-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
    • pGP1323 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
    • pGP1324 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References