NdoA

• Description: RNase EndoA, MazF family toxin, UACAU-specific mRNA interferase
  
  

Gene name	ndoA
Synonyms	ydcE, mazF
Essential	no
Product	mRNA interferase
Function	unknown
Gene expression levels in SubtiExpress: ndoA
Interactions involving this protein in SubtInteract: NdoA
MW, pI	12 kDa, 4.892
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	ndoAI, rsbR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Rnases, toxins, antitoxins and immunity against toxins

The gene

Basic information

• Locus tag: BSU04660

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: specifically cleaves a five-base sequence, UACAU PubMed

• Protein family: pemK family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: the activity is inhibited by the interaction with NdoAI PubMed

• Interactions:
  • NdoA(2)-NdoAI(2)-NdoA(2) PubMed
  • the active protein forms dimers PubMed

• Localization:

Database entries

• Structure:
  • 1NE8 PubMed
  • 4MDX (NdoA-RNA) PubMed
  • 4ME7 (NdoA-NdoAI complex) PubMed

• UniProt: P96622

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ndoAI-ndoA PubMed

• Expression browser: ndoA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • An antisense RNA is predicted for ndoA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Dhirendra K Simanshu, Yoshihiro Yamaguchi, Jung-Ho Park, Masayori Inouye, Dinshaw J Patel
Structural basis of mRNA recognition and cleavage by toxin MazF and its regulation by antitoxin MazE in Bacillus subtilis.
Mol Cell: 2013, 52(3);447-58
[PubMed:24120662] [WorldCat.org] [DOI] (I p)

Yojiro Ishida, Jung-Ho Park, Lili Mao, Yoshihiro Yamaguchi, Masayori Inouye
Replacement of all arginine residues with canavanine in MazF-bs mRNA interferase changes its specificity.
J Biol Chem: 2013, 288(11);7564-7571
[PubMed:23378533] [WorldCat.org] [DOI] (I p)

Xiangli Wu, Xiuhong Wang, Karl Drlica, Xilin Zhao
A toxin-antitoxin module in Bacillus subtilis can both mitigate and amplify effects of lethal stress.
PLoS One: 2011, 6(8);e23909
[PubMed:21897862] [WorldCat.org] [DOI] (I p)

Jung-Ho Park, Yoshihiro Yamaguchi, Masayori Inouye
Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase.
FEBS Lett: 2011, 585(15);2526-32
[PubMed:21763692] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Shivangi Agarwal, Shivani Agarwal, Rakesh Bhatnagar
Identification and characterization of a novel toxin-antitoxin module from Bacillus anthracis.
FEBS Lett: 2007, 581(9);1727-34
[PubMed:17416361] [WorldCat.org] [DOI] (P p)

Olivier Pellegrini, Nathalie Mathy, Arhonda Gogos, Lawrence Shapiro, Ciarán Condon
The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.
Mol Microbiol: 2005, 56(5);1139-48
[PubMed:15882409] [WorldCat.org] [DOI] (P p)

Arhonda Gogos, Haiyan Mu, Fabiana Bahna, Carlos A Gomez, Lawrence Shapiro
Crystal structure of YdcE protein from Bacillus subtilis.
Proteins: 2003, 53(2);320-2
[PubMed:14517982] [WorldCat.org] [DOI] (I p)